ISKrh7
- Family IS30
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP097204 | ND | Kocuria rhizophila | Kocuria rhizophila Kocuria rhizophila Noodlococcus Kocuria indica CE7 |
DNA section
IS Length : 1450 bp
Ends
IR Length : 22/26
IRL : CGGATTCAACTGGTCGTCGCAACACCTCGATCGGGAGGTGTCAGATGGTT
IRR : CGGGTTCAACCGGTGGTTGCAACACCGGCTTGTTGGAGCAACAGTAGCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCGACGCGGGGTC | TCAA | TTCACGTCCATTCGCT | 4 |
| ACAGCGACGCGGGGTC | TCAA | TTCACGTCCATTCG | 4 |
| ACAGCGACCGCGGCAG | TCAA | TTTCGAGCCCGGAA | 4 |
DNA sequence
CGGATTCAACTGGTCGTCGCAACACCTCGATCGGGAGGTGTCAGATGGTTCGTCGTCAGCAGGCAGCAGATCGGGCGGAGCGACCGAAGCTTCGGTCCCC
TGGGCATCCGAAGTTCCAACGGCATGTCGAGGCGGCGTTCTGGGCGGAGATCGCCAAGGGGCTATTGCCCATCGAGGCTGCTGCCGTGGTCGGCGTGGCG
CAAGCTGTCGGTCAGCGATGGTTCCGCCACGCTGGCGGCATGCCTCCATTCGATCTGAAGTTCACGCCAACCGGACGCTACCTGTCGTTCGCTGAGCGTG
AGGAGATCGCATTCCTGCGTGCTCAGGGACGCGGCGTGCGCGAGATCGCTCGAACTCTAGGCCGTAACCCGGGGACGATCTCCCGTGAGCTGCGCCGGAA
TGCGGCCGTCCGCTACGGAAGTCGCGGGTATCGAGCGTCGGTTGCGCAGTGGAAGGCCGACATGGCCGCGAAACGCCCGAAGGCGGCGAAGCTGGTCACG
AACGTGCGGTTGCATACCTACGTCCAGGACCGATTATCGGGGCAGATCGCCACGCCTGATGGCAAGGTGATAATCGGTCCCGAGCCCCCACGGTTCACGG
GGATCAACAAGCCCCACCGTAAGCACCGCGGGTGGTCCACGGCGTGGAGTCCGGAGCAGATCAGCAACCGGCTCAAGGTCGACTTCCCCGATGATGAGTC
CATGCGCATCAGTCACGAGGCGATCTACCAATCCCTCTACATTCAGGGCCGCGGCGCGCTCAAACGCGAGCTTGTCTGGTGTCTGCGCACGGGCCGGGCG
CTGCGCGCGCCGCGGGAACGGTCACGCCGCAAGACTTGGGCGCACGTCACTCCTGGAACGCTGATCAGCGAACGTCCCGCAGAAGCCGGAGACCGAGCTG
TTCCCGGCCATTGGGAGGGTGACCTGCTGATTGGGCTCGAGCGCTCAGCGATTGGCACCGTCGTCGACCGCACGACCAGGTTCACGATGCTGGTCCACCT
CCCCCGTGAGAAGGGATACCGACACAAGGAAACACCCAAGAACGGCCCCGCGCTGGCCGGCTATGGCGCGATCACGATGAAGAACGCGCTGGCGAGCACG
ATGTCGACGCTGCCCGCCCAACTGGCGAGGTCGTTGACCTGGGATCGCGGCAAGGAGATGTCCGCTCACGCACAGTTCACGGTCGAGACGGGCATCCCGG
TGTTCTTCGCCGATCCGCAGTCCCCCTGGCAACGCGGCACGAACGAGAACACCAACGGACTACTGCGCCAGTACTTCCCCAAGGGCACTGACCTGTCGAG
CTGGACGGCAGAGGAGATTGAAGCCGTCGCTCACGCACTCAACACAAGGCCCCGCAAGACCCTCGGCTGGAGAACCCCTGCCGAAGCCTTCAACGAGCAG
CTACTGTTGCTCCAACAAGCCGGTGTTGCAACCACCGGTTGAACCCG
TGGGCATCCGAAGTTCCAACGGCATGTCGAGGCGGCGTTCTGGGCGGAGATCGCCAAGGGGCTATTGCCCATCGAGGCTGCTGCCGTGGTCGGCGTGGCG
CAAGCTGTCGGTCAGCGATGGTTCCGCCACGCTGGCGGCATGCCTCCATTCGATCTGAAGTTCACGCCAACCGGACGCTACCTGTCGTTCGCTGAGCGTG
AGGAGATCGCATTCCTGCGTGCTCAGGGACGCGGCGTGCGCGAGATCGCTCGAACTCTAGGCCGTAACCCGGGGACGATCTCCCGTGAGCTGCGCCGGAA
TGCGGCCGTCCGCTACGGAAGTCGCGGGTATCGAGCGTCGGTTGCGCAGTGGAAGGCCGACATGGCCGCGAAACGCCCGAAGGCGGCGAAGCTGGTCACG
AACGTGCGGTTGCATACCTACGTCCAGGACCGATTATCGGGGCAGATCGCCACGCCTGATGGCAAGGTGATAATCGGTCCCGAGCCCCCACGGTTCACGG
GGATCAACAAGCCCCACCGTAAGCACCGCGGGTGGTCCACGGCGTGGAGTCCGGAGCAGATCAGCAACCGGCTCAAGGTCGACTTCCCCGATGATGAGTC
CATGCGCATCAGTCACGAGGCGATCTACCAATCCCTCTACATTCAGGGCCGCGGCGCGCTCAAACGCGAGCTTGTCTGGTGTCTGCGCACGGGCCGGGCG
CTGCGCGCGCCGCGGGAACGGTCACGCCGCAAGACTTGGGCGCACGTCACTCCTGGAACGCTGATCAGCGAACGTCCCGCAGAAGCCGGAGACCGAGCTG
TTCCCGGCCATTGGGAGGGTGACCTGCTGATTGGGCTCGAGCGCTCAGCGATTGGCACCGTCGTCGACCGCACGACCAGGTTCACGATGCTGGTCCACCT
CCCCCGTGAGAAGGGATACCGACACAAGGAAACACCCAAGAACGGCCCCGCGCTGGCCGGCTATGGCGCGATCACGATGAAGAACGCGCTGGCGAGCACG
ATGTCGACGCTGCCCGCCCAACTGGCGAGGTCGTTGACCTGGGATCGCGGCAAGGAGATGTCCGCTCACGCACAGTTCACGGTCGAGACGGGCATCCCGG
TGTTCTTCGCCGATCCGCAGTCCCCCTGGCAACGCGGCACGAACGAGAACACCAACGGACTACTGCGCCAGTACTTCCCCAAGGGCACTGACCTGTCGAG
CTGGACGGCAGAGGAGATTGAAGCCGTCGCTCACGCACTCAACACAAGGCCCCGCAAGACCCTCGGCTGGAGAACCCCTGCCGAAGCCTTCAACGAGCAG
CTACTGTTGCTCCAACAAGCCGGTGTTGCAACCACCGGTTGAACCCG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1398 bp | 465 aa | 47 | 1444 | + | No |
Chemistry : DDE
ORF sequence :
MVRRQQAADRAERPKLRSPGHPKFQRHVEAAFWAEIAKGLLPIEAAAVVGVAQAVGQRWFRHAGGMPPFDLKFTPTGRYLSFAEREEIAFLRAQGRGVRE
IARTLGRNPGTISRELRRNAAVRYGSRGYRASVAQWKADMAAKRPKAAKLVTNVRLHTYVQDRLSGQIATPDGKVIIGPEPPRFTGINKPHRKHRGWSTA
WSPEQISNRLKVDFPDDESMRISHEAIYQSLYIQGRGALKRELVWCLRTGRALRAPRERSRRKTWAHVTPGTLISERPAEAGDRAVPGHWEGDLLIGLER
SAIGTVVDRTTRFTMLVHLPREKGYRHKETPKNGPALAGYGAITMKNALASTMSTLPAQLARSLTWDRGKEMSAHAQFTVETGIPVFFADPQSPWQRGTN
ENTNGLLRQYFPKGTDLSSWTAEEIEAVAHALNTRPRKTLGWRTPAEAFNEQLLLLQQAGVATTG
IARTLGRNPGTISRELRRNAAVRYGSRGYRASVAQWKADMAAKRPKAAKLVTNVRLHTYVQDRLSGQIATPDGKVIIGPEPPRFTGINKPHRKHRGWSTA
WSPEQISNRLKVDFPDDESMRISHEAIYQSLYIQGRGALKRELVWCLRTGRALRAPRERSRRKTWAHVTPGTLISERPAEAGDRAVPGHWEGDLLIGLER
SAIGTVVDRTTRFTMLVHLPREKGYRHKETPKNGPALAGYGAITMKNALASTMSTLPAQLARSLTWDRGKEMSAHAQFTVETGIPVFFADPQSPWQRGTN
ENTNGLLRQYFPKGTDLSSWTAEEIEAVAHALNTRPRKTLGWRTPAEAFNEQLLLLQQAGVATTG
Blast result :
Comments
ISKrh7 is 85% aa similar to ISKrh5.
ISKr7 was found in the chromosome of the laboratory contaminant Kocuria rhizophila Noodlococcus. ISKhr7 is inside a 1,357 bp sequence that is bounded by 28 bp imperfect inverted repeats and appears to be an incomplete IS3 family element.
Two copies of ISKrh7 are present in the chromosome of K. indicus CE7, which was isolated from human skin. The copies in CE7 are identical to one another but differ from the copy in Noodlococcus by one SNP. One ISKrh7 in CE7 is flanked by the same sequence as the ISKrh7 in Noodlococcus.
Additionnal GenBank accessions : CP035504.
There is an empty site in Kocuria indica strain CE7 CP035504.
ISKr7 was found in the chromosome of the laboratory contaminant Kocuria rhizophila Noodlococcus. ISKhr7 is inside a 1,357 bp sequence that is bounded by 28 bp imperfect inverted repeats and appears to be an incomplete IS3 family element.
Two copies of ISKrh7 are present in the chromosome of K. indicus CE7, which was isolated from human skin. The copies in CE7 are identical to one another but differ from the copy in Noodlococcus by one SNP. One ISKrh7 in CE7 is flanked by the same sequence as the ISKrh7 in Noodlococcus.
Additionnal GenBank accessions : CP035504.
There is an empty site in Kocuria indica strain CE7 CP035504.
References
1] R. Moran (2022) Direct submission.
2] Ho,S.F., McCallum,G.E., Moran,R.A., McInnes,R.S., Wildsmith,A.J., Nicholls,S.M., Quick,J. and van Schaik,W. (2022) Direct GenBank submission.
2] Ho,S.F., McCallum,G.E., Moran,R.A., McInnes,R.S., Wildsmith,A.J., Nicholls,S.M., Quick,J. and van Schaik,W. (2022) Direct GenBank submission.