ISAbc2

  • Family IS1202
  • Group ISTde1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
RPON01000073 ND Acidobacteria bacterium
Acidobacteria bacterium isolate bin.240
DNA section
IS Length : 1556 bp

Ends


IR Length : 41/53

IRL : TGTTGCGCCTCGATGATAATGTCCCCTTCCCACCTCATTAGAAATGTCCC
IRR : TGTCAACCCTCAATAGAAATGTCCCCTTCCTTCCCTCGATAGAAATGTCC

Insertion site


Left flankDirect repeatRight flankDR Length
CCTCGTGACGTACGGCGAATGTCAAGAGGAGAGAGTTGAC0

DNA sequence

TGTTGCGCCTCGATGATAATGTCCCCTTCCCACCTCATTAGAAATGTCCCCTTCGCCGAGACCTCCGCTGAGGAGGACGGCGTTGGAAAGGACACCAATG
AGCGTGCGGGAGCTGAAGCGGATTGAGGTGTTCGCCCGGGTGAAGGCGGGGACCTTGAAAGTCAAGGATGCCGCCGTCCTGCTGGGGGTGTGTGAGCGCC
AGGGGAAGCGGCTGTGGCGCGAGTACCGGAGGAAAGGGCCAAGAGGGCTGCGGCACCGGAGTGCTGGGAAGCGGTCGAATCGAGCGAAGTCGACCGCCTG
GCGCCGGAAGGTGGTGGGACTCTACCGTCGGAAGTATGGCGGGAACGCGAAGCGGGACGAGGCGCCGTTTGGGCCCACGTTGGCGGCCGAGCATATGGGC
AAGGACGACCAGACGCCGGTCCATCCCGAAACGCTGCGACGGCTGTTGATCGCGGAGGGGCTGTGGGCACGGAAGCGCAAGGGCGTGGCGCATCGCCAGC
GTCGCGAGCGGAAGGCCCACTTTGGGGAGTTGGTCCAAGTCGACGGCAGCTTCCATGAGTGGCTGGAAGGCCGCGGGCCGCGCGGGTGCCTGATCCAGGC
GATCGACGACGCCACGAGCACGGCCGAGGGGCGCCTGTCGGACCAGGAGACGATCTGGGCGGCCGCGGCGGTATTGCGGCGATGGGTCGAGCGATACGGC
ATCCCGCACGCGCTCTATACGGACTGGAAGAACGTGTATCTGCGAGAGCCCACCGAGGCGGAGCGCGCCTCGGGATGCGTGCCCCGCACGCAGTTTGGGA
GGATGTGCGAGAAGCTCGGGGTCGACATCATTGGAGCCAGCTCGCCCCAGGCGAAGGGGCGGGTCGAACGCTCGCATGGGACATCCCAGGACCGTCTGGT
CAAGAAGCTGCGGCTCAAGGGCATCAGCGACCACGCCGCGGCCAATGCCTATCTCGCCCGCGAATACTACCCGGGTCACAATGCGCGATTTGCCCGGAGG
CCCGCGTCGGAGGAGGACTTCCACCTCCCGGTGCCGCGAGGCCTGGACCTCGACGAGGTCTTCCGGCTCGAGACCGAACACACGCTCGGGAACGACTGGG
TGGTGCGTCACGACAACCGCTACTTCCAAGTGCAGCGCCAGAGTCGGTACGCGCCGGCGCGCAGCAGAGTGCTTGTCTGCGAGTACGAGGACGGAAGTCT
GGGGATTGAATACCGGGGGCAGAAGCTGGCTTTCGAGGAGATCCCGGCGCCGACGCCAGCGGCAGCTGGGAAGCCGGAGGCCGCCGGGACCAAGCGGTCC
AGCAAGCCGGGAGCGCAGCACCCCTGGCGGCGTCGCTACGGTGACATGCCGGACCGGCTGCCCTGGCCGAGCAGGCGACCCGAGGGCTGTGGAAATGACA
GCGGCGTGGACGCTCAGCGGACGAGCGTCCACCCCGCCTTGGAAACCTCGCGAAGTGCGCGAGATTCCCACATTCCCACAAGCCCGTAATCACAACCCTC
TTCAGGGGACATTTCTATCGAGGGAAGGAAGGGGACATTTCTATTGAGGGTTGACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1446 bp481 aa441489+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSPSPRPPLRRTALERTPMSVRELKRIEVFARVKAGTLKVKDAAVLLGVCERQGKRLWREYRRKGPRGLRHRSAGKRSNRAKSTAWRRKVVGLYRRKYGG
NAKRDEAPFGPTLAAEHMGKDDQTPVHPETLRRLLIAEGLWARKRKGVAHRQRRERKAHFGELVQVDGSFHEWLEGRGPRGCLIQAIDDATSTAEGRLSD
QETIWAAAAVLRRWVERYGIPHALYTDWKNVYLREPTEAERASGCVPRTQFGRMCEKLGVDIIGASSPQAKGRVERSHGTSQDRLVKKLRLKGISDHAAA
NAYLAREYYPGHNARFARRPASEEDFHLPVPRGLDLDEVFRLETEHTLGNDWVVRHDNRYFQVQRQSRYAPARSRVLVCEYEDGSLGIEYRGQKLAFEEI
PAPTPAAAGKPEAAGTKRSSKPGAQHPWRRRYGDMPDRLPWPSRRPEGCGNDSGVDAQRTSVHPALETSRSARDSHIPTSP

 

Blast result :
Comments
ISAbc2 is 60% aa similar to ISTde1.
References
1] Patricia Siguier (2020) Direct submission.
2] Dalcin Martins,P., Danczak,R.E., Roux,S., Frank,J., Borton,M.A., Wolfe,R.A., Burris,M.N. and Wilkins,M.J. (2018) Microbiome 6 (1), 138 .