ISAasp1

  • Family IS1202
  • Group IS1202
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP048000 ND Anaerocolumna sp.
Anaerocolumna sp. CBA3638
DNA section
IS Length : 1642 bp

Ends


IR Length : 18/26

IRL : TATGATTCATTTATGATAATGTCTTAGTGTACCACAAATGATTATGTCCC
IRR : TGTCAAGCGTTTTTGAAAATGTCCTAAAAAAGTTTTAATATAAGCACCAT

Insertion site


Left flankDirect repeatRight flankDR Length
ATCAAGATATACGTATGGTGTCAAACATTTTTTAAAATTAATACTTTAAGTGACTTTTCGACAATCG26

DNA sequence

TATGATTCATTTATGATAATGTCTTAGTGTACCACAAATGATTATGTCCCAAATAGTAGTAAGTATATTATAATAGAACCTCATGAGTAAGAAATGAGGT
GGCACAATCAGAAAGGTATTACTAACAATGGATGAAAACCATAAGTATGAAATTATTAAAAAGCTCGTTGATACTAACGGTAACAAGAAAACAGCAGCTC
TCAAAATCGGTTGTTCAGATCGTCATATAAATCGGTTGATAAAAGATTACAAAGAAGAAGGTAAAGCTTTCTTTGTTCATGGCAACCGAGGTAGAAAACC
CGCTATCACTCTACCTACAGATACCAAACAGTTAATCTTGGACTTATATCGTAATAAATACTATGACTGCAACCTAACTCATTTTTCTGAATTACTTGCT
GAAAAAGAAAATATCAAGGTATCCGTCAGTACTATTTCTTCTACCCTTAGAAAGGAATTCATCCTTTCACCTAAAGCAAAGCGTTCTACCAAAAAGGAAG
TAAAAAAAGAATTAAAGGCTCTTCAATCAAAGACTAAATCGAAAAAGAAAGCAGCCGCATTCCAAAGCTCCATCCTGGAACTTGAGGATTCCCATCCAAG
GCGCCCTAGGTGTGCCTATTTCGGCGAAATGCTACAAATGGACGCTTCTCTGCATCATTGGTTTGGAGATACTAAAACACAGCTTCATATCGCTGTTGAT
GATGCAACTGGAGCAATTCTTGGTGCTTACTTTGATATGCAGGAAACCCTAAATGGCTACTATCATGTACTACATAAGGTTCTTAACTCTTACGGTATAC
CGTATCAGTTTTTTACTGATAATCGAACTGTTTTTGAATATAAAAAGAAACAGGAAACTTCTGTTGATAAGGATACCTTTACTCAGTTTGGTTATGCCTG
CAAACAATTAGGAATTGACATTCAGACATCGAGCGTTGCACAAGCAAAAGGGCGGGTTGAAAGAATGTTTGGCACCCTTCAATCCCGCCTTCCAATAGAG
CTACGCTTAGCCAATGTTTCCTCTATCGAGGAAGCAAATGAATTTCTAAACTCCTACATAAAGAAATTCAATAAGCAATTCGCTTTACCTATAGATAGTA
TCAAATCTGTGTTCGAAACGCAACCAGATAATGAAAAAATCAATCTTACACTGGCTGTGCTGTCTTCTCGCAAGATAGATAACGGGAGTTGTTTAAAGTA
TCAAAACCAGTATTACCTTCCCGTAAATGGCCAGGGTATTGCTGTACATCACAGAAAAGGTACTACCGCAATGGTGATAAAAGCATTTAATGGCGATCTC
TATAGCTGTATTGGTGAGCAGGTCTACGCACTTGAATTACTGCCAGAACATAAACTTTCCTCGAAAGCATTTGATTTAGCAACTATACCTGATGCGCCCA
AGAAAAAATACATACCACCAATGAGCCATCCATGGAAACAGGCATCATTTGAAAAATATGCAAAAAGCCAGTTACATAGAAAAGAAGTAGCTTAACTAAC
ATTTAGAAATCGGCAGGTTTATTAAAGTTACCCTAAAACCGACTCAGTAATTAATATTCACCTGAAATATAACCCAAGCTCAGCACCAGAACATGGTGCT
TATATTAAAACTTTTTTAGGACATTTTCAAAAACGCTTGACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1368 bp455 aa1281495+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDENHKYEIIKKLVDTNGNKKTAALKIGCSDRHINRLIKDYKEEGKAFFVHGNRGRKPAITLPTDTKQLILDLYRNKYYDCNLTHFSELLAEKENIKVSV
STISSTLRKEFILSPKAKRSTKKEVKKELKALQSKTKSKKKAAAFQSSILELEDSHPRRPRCAYFGEMLQMDASLHHWFGDTKTQLHIAVDDATGAILGA
YFDMQETLNGYYHVLHKVLNSYGIPYQFFTDNRTVFEYKKKQETSVDKDTFTQFGYACKQLGIDIQTSSVAQAKGRVERMFGTLQSRLPIELRLANVSSI
EEANEFLNSYIKKFNKQFALPIDSIKSVFETQPDNEKINLTLAVLSSRKIDNGSCLKYQNQYYLPVNGQGIAVHHRKGTTAMVIKAFNGDLYSCIGEQVY
ALELLPEHKLSSKAFDLATIPDAPKKKYIPPMSHPWKQASFEKYAKSQLHRKEVA

 

Blast result :
Comments
ISAasp1 is 83% aa similar to ISClsp7.
References
1] Patricia Siguier (2020) Direct submission.
2] Kim,J. and Roh,S.W. (2020) Direct GenBank submission.