ISAbc3

  • Family IS1202
  • Group ISTde1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
QHYP01000202 ND Acidobacteria bacterium
Acidobacteria bacterium isolate gp2 AA85
DNA section
IS Length : 1383 bp

Ends


IR Length : 24/30

IRL : TGTCGCCGCAAAATGAGAATGTCCGGTTTGAGCAAAGTAGAAATGTCCGG
IRR : TGTCAACGCAAAGTAGAAATGTCCGCTTTTCTGCCATATTGAAATGTCCG

Insertion site


Left flankDirect repeatRight flankDR Length
AGAAGGGTCAACGCGCGAACTGGCGCCCGCGCGAACTGGCGCCGATTGGCTATTGTGA16

DNA sequence

TGTCGCCGCAAAATGAGAATGTCCGGTTTGAGCAAAGTAGAAATGTCCGGTTTCATGGGGGCCCGGAGGTCCCATGGAAACGGAGCGCATCGCCTTGAGT
CAACGAGAACGGGACCGGCTGAGAGTGTTACACGAGGTAAAGCAGAAACAGATCACACAGATCGCAGCTGCCGGGCGGCTGAAGATCACCGCTCGTCAGG
TGCGTCGCCTGCTGCTCCGTCTGCGGGAGCAGGGAGACCGGGGGCTGATTCACGGATTACGTGGACGGCCCTCGAACCGCAGGTTGGCTCCCGTGTTCGA
GCAGAAGATTCTGGCGCGGGTGCGCCAACGTTATGCGGACTTCGGGCCCACCCTGGCCGCCGAACACTTGGCCCAGGAGGGGTTGCGGGTGAGCCGCGAG
ACGCTGCGGAAGTGGATGACCAAGGCCAGCTTGTGGCGCCCGCGCTCCCAGCGCGTGAAGATTATCCATGTGTGGCGTGAGCGCCGGGCCAGTTTCGGAG
AGTTGGTGATGCAGGATAGCTCCCCGTTTCGCTGGTTAGAGGAGCGTGGCCCGGCATGTCAGCTGATCGCCGTGATCGATGATGCCACGAGTCGCCTGTG
GGCACGTTTCACCGAACAGGACACCACGGAAGAGAACCTGCGAACCTTCGGGGGCTGGTTGCGCCGCTATGGACGTCCGCTCGCTCATTACACCGATAAA
AACAGCATCTTTCGCATGGCCGGCGCAGCAGCCATCCGGGAACAACTGCGAGGAGAAGGGGCACGTTCGCAGTTCGGGAGGGCGCTCCGCGAACTAGGGA
TCGAATGGATCGCGGCGCACAGCCCCCAGGCGAAAGGACGGATCGAGCGACTGTTTGAAACTCTGCAGGACCGGCTGGTGAAGGAGATGCGCTTGGCGGG
GATCGCTAGCCTCGAAGCAGCCAATCACTTCTTGGAGTTGCGTTTCCTGCCGGTGTGGGAACAACGCTTCACCGTGGCACCTCGCAACCCACGCAATGCT
CATCGACGACTGGGGCAAGAACATCGCCTGGAAGAAATCCTGAGTGTGCGCGTGGTCCGCAAGGTGGCAGAAGATCACACCGTCAGCTGGGAGGGAAACC
GTTGGGGTGTGCCACGGGAAGAAGTCTGCGCAGGTCTTCGCGGAGCGCAAGTCGAGATCGAACGGCGGCTGGATGGCTCGCACTGGTTGCGCTTCCGCGG
CCGTTACCTCCGCCTGCGGCACTGCCCGGAACCGGCGCCGCGCGCGGCAAGTCCTTCCGGCCTACGGCCTCCAGTCCTTGCCGCCAATCCCCTCTCAAGA
GTTTACAAAAGTGCTCCTCCGAACCACCCATGGCGGACATTTCAATATGGCAGAAAAGCGGACATTTCTACTTTGCGTTGACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1308 bp435 aa741381+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

METERIALSQRERDRLRVLHEVKQKQITQIAAAGRLKITARQVRRLLLRLREQGDRGLIHGLRGRPSNRRLAPVFEQKILARVRQRYADFGPTLAAEHLA
QEGLRVSRETLRKWMTKASLWRPRSQRVKIIHVWRERRASFGELVMQDSSPFRWLEERGPACQLIAVIDDATSRLWARFTEQDTTEENLRTFGGWLRRYG
RPLAHYTDKNSIFRMAGAAAIREQLRGEGARSQFGRALRELGIEWIAAHSPQAKGRIERLFETLQDRLVKEMRLAGIASLEAANHFLELRFLPVWEQRFT
VAPRNPRNAHRRLGQEHRLEEILSVRVVRKVAEDHTVSWEGNRWGVPREEVCAGLRGAQVEIERRLDGSHWLRFRGRYLRLRHCPEPAPRAASPSGLRPP
VLAANPLSRVYKSAPPNHPWRTFQYGRKADISTLR

 

Blast result :
Comments
ISAbc3 is 59% aa similar to ISAmba1.
References
1] Patricia Siguier (2020) Direct GenBank submission.
2] Crits-Christoph,A., Diamond,S., Butterfield,C.N., Thomas,B.C. and Banfield,J.F. (2018) Nature.