ISAchy1

  • Family IS1202
  • Group ISTde1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ACJX03000001 ND Acetomicrobium hydrogeniformans
Acetomicrobium hydrogeniformans ATCC BAA-1850
DNA section
IS Length : 1549 bp

Ends


IR Length : 17/24

IRL : TGTGGTTAATCCGTGATAATGTCATGTTGAACCTAATCTGAGAAAATGTC
IRR : TGTCAATAACCCATAAAAATGTCACCTAAACAACATAGTTTTATTCAGTA

Insertion site


Left flankDirect repeatRight flankDR Length
GTGCTGTCAACATAGAGTAAAAATTGAGCTTAGAAAA17

DNA sequence

TGTGGTTAATCCGTGATAATGTCATGTTGAACCTAATCTGAGAAAATGTCACCCATGAGAGAAGAAATATTCAACATGACAAAACAGGAAATGAAAAAGC
TCAGGGTCATTGACTCGGCTATTTCAGGGAATGTAACAGTTAAAGAGGCAGCGGGATTGCTTGGCCTAAGCGAACGTCAAGTGTTGAGGCTAAAGAAAGG
GGTATTGAAACAAGGAGCCGCGTTCATCACCCACAAAAATAAAGGTCGTAAGCCATCCCATGCAATTTCAGACACAATGAAAGATAAAATTGTAGCATTG
AAGCAATCGGATAGCTATCGAAACGCCAATTTCACTCATTTCAAGGAAATTCTTTCGGGGCAAGAGGGTATTAATATAAGTTACTCAAGCGTGTACCGGG
TCCTTAGCCAAGCCGGTATTTGTAGCTCCAAGAAAAAGCGAAGGAGTAAAAACCACCATCGCCGCAAGAGGAAAGAAAAAGAAGGCCTTCTGGTCCAAAT
CGATGCATCTCCACATGCTTGGTTCAATGAAACCATACACTCCCTACATGGTGCGATTGATGATGCCACTGGAGAAATACTTGCTTTACATTTTGAGAAA
AACGAATGCCTTAGGGGTTATTTTGAAATAATGGAACAAATGATTGAGCGTAAAGGGATCCCTGTTAACATATACAGCGATCGCCACACGATTTTTAAGT
CCCCTAAGAAAGAGGAACCAAGGATTGAACAGCAGCTAAAAGGTAAGCAAACGAATTTAACGCAGTTCGGTGAGGCAATGGAGGCACTTGAGATAAACCT
GATATTTGCTAGATCACCACAGGCAAAAGGACGTATTGAAAGGCTATGGGAAACGCTGCAAAATAGGTTGCCGGTTGAGTTTAAGATCCATGGTATTACA
TCAATGGAAACAGCCAATGAGTTTTTAATCGGCTATATCGATAAGTTTAACGAGCAGTTTACCGTGATACCAAAAGACCCCCAAGGCGCCTTCCGTGAAC
TTGAAGATGGTGTTGATCTTAGCACTGTCCTTTGTATCAAGGAGCAACGTGTGGTTTCTGATGGAAGCGGATTCTCTTATGGGGGCAAGTACTACCAGGT
AATGGGTAAGGAAAAACCGGTGGGGTTGCCCCGGGGGGTGACAATTACTGTGTTAAATAGCCATAAACGAGAGCTAAAAGTGATGTATAACGGGGTGGTA
TACGGAACCAACTACATGGCTCAAAAACCCAAGGAAAAGGCTTTACAGGAGCCATGCAAGGAGACACAAAGGATTGTAGCACGGCCAGATGATGACCACC
CTTGGAGGATACCGAAACAAAAAAGACCCAGGCTTTCCTATGATGAATCGGATAGTAAGCTCCTTGAAGCTCTCTTCAATTCGTCACGTGCCTGGGCATA
GAAACCTTCTTATAACCAGCTTACCCTTATTGGCCCGGTTTATCAATGGCATTTTTGCTTAAGGCCGGACTCCGTCGAAAACGACTAAGGTGACATTTTT
ACTGAATAAAACTATGTTGTTTAGGTGACATTTTTATGGGTTATTGACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1347 bp448 aa551401+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MREEIFNMTKQEMKKLRVIDSAISGNVTVKEAAGLLGLSERQVLRLKKGVLKQGAAFITHKNKGRKPSHAISDTMKDKIVALKQSDSYRNANFTHFKEIL
SGQEGINISYSSVYRVLSQAGICSSKKKRRSKNHHRRKRKEKEGLLVQIDASPHAWFNETIHSLHGAIDDATGEILALHFEKNECLRGYFEIMEQMIERK
GIPVNIYSDRHTIFKSPKKEEPRIEQQLKGKQTNLTQFGEAMEALEINLIFARSPQAKGRIERLWETLQNRLPVEFKIHGITSMETANEFLIGYIDKFNE
QFTVIPKDPQGAFRELEDGVDLSTVLCIKEQRVVSDGSGFSYGGKYYQVMGKEKPVGLPRGVTITVLNSHKRELKVMYNGVVYGTNYMAQKPKEKALQEP
CKETQRIVARPDDDHPWRIPKQKRPRLSYDESDSKLLEALFNSSRAWA

 

Blast result :
Comments
ISAchy1 is 69% aa similar to ISSsac1.
References
1] Patricia Siguier (2020) Direct submission.
2] Weinstock,G., Sodergren,E., Clifton,S., Fulton,L., Fulton,B., Courtney,L., Fronick,C., Harrison,M., Strong,C., Farmer,C., Delahaunty,K., Markovic,C., Hall,O., Minx,P., Tomlinson,C., Mitreva,M., Nelson,J., Hou,S., Wollam,A., Pepin,K.H., Johnson,M., Bhonagiri,V., Nash,W.E., Warren,W., Chinwalla,A., Mardis,E.R. and Wilson,R.K. (2012) Direct GenBank submission.