ISAnth1

  • Family IS1202
  • Group ISTde1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
DPBP01000033 ND Anaerolinea thermolimosa
Anaerolinea thermolimosa isolate UBA8781
DNA section
IS Length : 1467 bp

Ends


IR Length : 38/51

IRL : TGTTAGTGCAAAGTAGTAAAGTCCCCTTTGTGCAAAGTTAAAATGTCCCC
IRR : TGTCAATGCAAAATAGAAATGTCCCCTGAAATGCAAAATAGAGATGTCCC

Insertion site


Left flankDirect repeatRight flankDR Length
ATGGCCCGGGTAAGCGTCGACATGGCTCTT0

DNA sequence

TGTTAGTGCAAAGTAGTAAAGTCCCCTTTGTGCAAAGTTAAAATGTCCCCATGGGAGGGACGAGAAAGGGAATGGACGAACTACTAGCGATGAGTAAAGA
AGAGATCACCCGGTTAGAAACGATGCAACGGATAAAAGACAAGCGGCTAACGCAAGGGGAAGCCGCGCGGATACTGGGGCTGAGTGTACGACAGATCAAA
CGGCTGTATCGGGCATACAAAGGCGAAGGAGCGGCAGGGCTGGTGAGCCGAAGGCGGGGGAAGCCGAGCAACCATCGGCTGGATGGAGGAACGCGGCAGA
AAGCCCTGGACCTGCTGTATGAGCGTTATGCGGACTTTGGTCCGACGTTAGCGCATGAGAAGTTGAGCGAGCGGCATCAATTGAGGCTTTCGCGGGAAAG
TGTGCGCAAACTGATGATAGCTGAAGGGCTATGGAAGCCGCGGCGGGGGAAGAAAGCGCCAGTGCACCAGATGCGCGAACGGAGAGCGTGTTTAGGGGAG
TTAGTGCAAATCGATGGCTCGGATCATGCCTGGTTCGAAGAACGGGGTCCGCAGTGTACCCTGCTGGTGTTTATCGATGATGCGACGGGACAGTTGATGG
AACTGTGGTTTGTGCCCGATGAGACCTTTTTCGCCTACTGTGAGGCGACGCGCCACTACCTGGAGCGTTATGGCAAGCCGCTAGCCTTCTACAGCGACCG
TCATGGCATTTTTCGGGTCAATCAGCCCCGTCCTCTGGCAACCACCTCCGGGTTGACCCAATTTGGACGGGCCATGCAGGAACTGGAGATCCAGATCCTG
TGTGCCAGTACTCCCCAAGCCAAAGGACGCATTGAACGGGCGAACCAGACCCTGCAAGACCGCCTGGTGAAAGAACTGCGTTTACGGGGGATTTCTGACA
TGGCCACGGCGAATGCTTTCTTACCCGAGTTTCGGGAAGATTTCAACCGCCGTTTTGCAGTAGCCCCGCGCAGTCATCACAATGCCCATCGACCCTTGTT
ACCTGCCGAAAACCTGGACCTGATCCTGTCTCACCAGGAAACCCGTACCCTCTCCAAAAACCTGACCCTCCAGTTCAAGCACATGATCTACCAGATCCAA
TCCGAACGCCCAGACTATGCCTTACGCAATGCCCGGGTAACCGTTTGCGAGACCCCTCAAGGAGAAGTGACGATCCTCTACAAAAACCAGCCCTTGCCTT
ACTCCCTCTACCAGAAGGCGATCCGTCAAGCCGACATCGTGGATACCAAATCCCTCGATCGTCAGGTCAAGGCTCCACCGTCCCCTGCCCCCAATCACCC
CTGGCGCCGCTATGGTCACCACCTGAACGGGAAACCCATCGAAGAAGCTTCACCCCATGGCACTGACTAACATGGACCTCAACATCACATCTGGCATTGT
CTCACCACTCTTCATAGGGGACATCTCTATTTTGCATTTCAGGGGACATTTCTATTTTGCATTGACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1320 bp439 aa511370+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGGTRKGMDELLAMSKEEITRLETMQRIKDKRLTQGEAARILGLSVRQIKRLYRAYKGEGAAGLVSRRRGKPSNHRLDGGTRQKALDLLYERYADFGPTL
AHEKLSERHQLRLSRESVRKLMIAEGLWKPRRGKKAPVHQMRERRACLGELVQIDGSDHAWFEERGPQCTLLVFIDDATGQLMELWFVPDETFFAYCEAT
RHYLERYGKPLAFYSDRHGIFRVNQPRPLATTSGLTQFGRAMQELEIQILCASTPQAKGRIERANQTLQDRLVKELRLRGISDMATANAFLPEFREDFNR
RFAVAPRSHHNAHRPLLPAENLDLILSHQETRTLSKNLTLQFKHMIYQIQSERPDYALRNARVTVCETPQGEVTILYKNQPLPYSLYQKAIRQADIVDTK
SLDRQVKAPPSPAPNHPWRRYGHHLNGKPIEEASPHGTD

 

Blast result :
Comments
ISAnth1 is 67% aa similar to ISThsi1.
References
1] Patricia Siguier (2020) Direct submission.
2] Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A., Chaumeil,P.A. and Hugenholtz,P. (2018) Nat. Biotechnol.