ISArba2

  • Family IS1202
  • Group ISTde1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
DOWH01000587 ND Armatimonadetes bacterium
Armatimonadetes bacterium isolate UBA10553
DNA section
IS Length : 1504 bp

Ends


IR Length : 48

IRL : TGTCGGGGCAACGTGATAATGTCCGGTCGTGGCAAAGTAGAAATGTCCTC
IRR : TGTCGGGGCAACGTGATAATGTCCGGTCGTGGCAAAGTAGAAATGTCCGG

Insertion site


Left flankDirect repeatRight flankDR Length
GGGATCTTCCCGCAGGTCCGAGCTTGAGGAT0

DNA sequence

TGTCGGGGCAACGTGATAATGTCCGGTCGTGGCAAAGTAGAAATGTCCTCTCCAGCGGGGCGCGCCCCGCGACGCGGCGAACAGGGAGGGCATGAAGCTG
ATAATGAGCACGAAAGAGCGAGATCGACTGGCCGCGATCAGAGCAGTTGTTGCCGGACAGATAACCCAGAAGCAGGCCGCTGAGCAAGCCGGGATCAGCG
CACGGCAGATGCGCCGCGTCGTCGCCCGCTACCGGAAGGAGGGCGACGCCGGGCTGATCCACAGATCCCGTGGCCGCCCGTCGAACCGCCACTATTCGGA
CGAGGTGCAGGCGCGTGCGGTTGAACTGATTACGGAGCACTACCGCGGCTTCGGCCCGACGCTGGCCGCGGAGAAGCTCGCCGAACGTGACGGCATCATC
GCCTCCAAAGAGACGGTGCGTCAATGGATGATCGAGGCCGAACTGCACACGCCCAAGCCCCGGAAGCTCGAGCACCGTCAGTGGCGCGAGCGCAAGCCAT
GTTGGGGCGAACTCCTGCAGGTGGATGGTTCAAAGCATCCCTGGCTGGATGGCAGAGGGCCACTGCAGCCGGATCTGATCTCTGCGATCGATGATGCCAC
CGGACGCGTGCTGCTGCAATTCGCGCCCGCCGAGAGCACCGAGGCGGTGATGCGCCTGCTGCATGAGTACATCAGCCTCTACGGACGCCCGCTGGCCATC
TACGCCGACCGGCACAGCATCTATCAAACGAGCCGCCAGGCCTCCGTAGAGGAGCAACTGCAGGGCCGGGAGGCTGAGACACAGCTTGGGCGCGCCCTGC
GGGAGCTGGACATCAAGTACATCCCAGCCTACTCGCCGCAGGCCAAAGGGCGGGTGGAACGCAGCTTCGGCACCCTCCAGGACCGGCTCGCCAAAGAGCT
GCGCCTGGAGGGGATCTGCGATATCGACAGCGCGAACCGCTTTCTCAAAGAGCGCTTCATCGGCCAGCACAATGAGCGTTTCGCCGTGCAGCCGACCAGC
GATCACGATGCACATCGCTCCGCCGAGGGCTTCGACCTGGACGCGATCCTCAGCCACCAGGAGACGCGGGTTGTGACCAATGACTACACCATCAGCTACT
ACGGCGCGCGCCTGCAGATCGCGCGCGCGAACGTCGCCGGCCTGCGCGGGAGCAAGGTCATCGTGGAGCGACGGCTCGACCGCTCGATCCACGTGCGCTT
CAACGGCGCATATCTTACTGTGACTGAGTTGCCACCGGTCCAGCCGAAGGCAACTCCCCCAAGACCGCACAAGCGGCGCGAGCCAAGCGTGGTCATCCCA
GCCGCCGATCATCCCTGGCGCGGAGACTACCGTAAGATGCCGGATGGGCCGATCTACCCATGACCGGGCGCGCCGCCCCCCTCGGGGGGAGCAGGGCAAC
AGCTACGGCTACGACAAGGACTACGACCACGGCCCACTTAGGGGACCACAATAACCGGACATTTCTACTTTGCCACGACCGGACATTATCACGTTGCCCC
GACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1272 bp423 aa921363+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKLIMSTKERDRLAAIRAVVAGQITQKQAAEQAGISARQMRRVVARYRKEGDAGLIHRSRGRPSNRHYSDEVQARAVELITEHYRGFGPTLAAEKLAERD
GIIASKETVRQWMIEAELHTPKPRKLEHRQWRERKPCWGELLQVDGSKHPWLDGRGPLQPDLISAIDDATGRVLLQFAPAESTEAVMRLLHEYISLYGRP
LAIYADRHSIYQTSRQASVEEQLQGREAETQLGRALRELDIKYIPAYSPQAKGRVERSFGTLQDRLAKELRLEGICDIDSANRFLKERFIGQHNERFAVQ
PTSDHDAHRSAEGFDLDAILSHQETRVVTNDYTISYYGARLQIARANVAGLRGSKVIVERRLDRSIHVRFNGAYLTVTELPPVQPKATPPRPHKRREPSV
VIPAADHPWRGDYRKMPDGPIYP

 

Blast result :
Comments
ISArba2 is 60% aa similar to ISElba1.
References
1] Patricia Siguier (2020) Direct submission.
2] Parks,D.H., Chuvochina,M., Waite,D.W., Rinke,C., Skarshewski,A., Chaumeil,P.A. and Hugenholtz,P. (2018) Nat. Biotechnol.