ISKpn1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF345899 | Y | Klebsiella pneumoniae | Klebsiella pneumoniae MGH78578 |
DNA section
IS Length : 1445 bp
Ends
IR Length : 22/24
IRL : TGGACTGCACCCCAAAAGTTGGACACCCAACTGAGTAAGGTGCAATTTTA
IRR : TGGACTGACCCCATAAAGTTGGATGGTTCATATTAAGCGGCTCTCAGAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCCGGCGGCG | C/CTG | GCTTGCCGGG | 5 |
DNA sequence
TGGACTGCACCCCAAAAGTTGGACACCCAACTGAGTAAGGTGCAATTTTATGGCTAAACCAAAGTATTCCCCTGAAACAAAACTGGCTGTGGTTAATCAT
TATTTGTCCGGTAAAGACGGAGAACAGAGTACAGCCGACCTTTTTGGTATTGAAAGAACATCTGTCCGTCGCTGGGTCAGGGCATGGCAGTTCCACGGTG
CAGAAGGCCTGACTGCAAAAAATAATCATTATTCCGATGAATTTAAACTCGTGGTCGTCCGGGCGGTTATCAGTGACCGCCTGACGATGCGTGAAGCGGC
TGCCCGGTTTAATCTCTCCGCAGAAATACTTGTCCGGCGCTGGCTCGACGTGTACAACGATGCCGGAGCGGAAGGTCTTTTAAACATGCAATGCGGACGG
CCCGGACAAATGACAAAGCCAAAAAATATCCCACCACTGACAGATAAAGAGCTGGAAAAACTCTCCCCCGAAGAGCTCAGGGCCGAACTGCGTTATCTGC
GGGCAGAGAATGCCTATCTAAAAAAGTTGAAAGCCTTGGTTCAGAGCGAAAAAAATGGCAAAAAGCCCTGATAATCAGTGAACTAAGGCATGAACACGCT
CTGCGGGACCTTCTGCGGGCGGCCGGTATGTCCCGTAGCACGTGGTATTACAATATGAATGCACTGAAGCAAGGGGACAGGTATGCGGGTCTTAAAGAGA
ACATCAGGAAGATATACCACTATCACAAAGGTCGTTATGGCTACCGCAGGATCACGCTCGCACTAAGAAAACAGGGGCTGCGGATAAACCATAAAACAGT
GCAGCGGCTGATGGCAGAACTGTCACTCCGGTCTGTGATAAGGGCGAAAAAATATCGTGCCTGGAAAGGGAGAACGGGCGAGGCCGCGCCCAATATCCTG
AGCCGGAACTTCGGTGCGTCAAAAGCCAACGAAAAATGGGTAACAGATGTGACAGAGTTCCCGGTACAGGGAAAAAAGCTTTACCTGTCGTCAGTTCTCG
ATCTGTTTAACCGGGAGGTTATCGCCTACAGCCTGTCGGAAAGGCCGGTGATGGAGATGGTGAATACGATGCTGGACGGTGCGTTCCCGAAGCTCAGACC
GGGAGATGCTCCGCTGCTGCACTCGGATCAGGGCTGGCATTACAGGATGAGGAGCTATCAGGAACGTTTAAAGGCGCATGGGATGACGCAGAGTATGTCG
CGTAAAGGAAACTGCCTGGATAATGCAGTGATGGAAAACTTCTTCGGGACCCTGAAATCGGAGTGTTTTTATCTGAGGGAGTTCAGGAGTGTCAGTGCGC
TAAGAAAAGCCGTAGAGGACTATATCCATTACTACAACAACGAGCGGATAAGCCTGAAATTAAAAGGCCTGAGTCCGGTAGAGTACCGAACCCAGGCTCT
GAGAGCCGCTTAATATGAACCATCCAACTTTATGGGGTCAGTCCA
TATTTGTCCGGTAAAGACGGAGAACAGAGTACAGCCGACCTTTTTGGTATTGAAAGAACATCTGTCCGTCGCTGGGTCAGGGCATGGCAGTTCCACGGTG
CAGAAGGCCTGACTGCAAAAAATAATCATTATTCCGATGAATTTAAACTCGTGGTCGTCCGGGCGGTTATCAGTGACCGCCTGACGATGCGTGAAGCGGC
TGCCCGGTTTAATCTCTCCGCAGAAATACTTGTCCGGCGCTGGCTCGACGTGTACAACGATGCCGGAGCGGAAGGTCTTTTAAACATGCAATGCGGACGG
CCCGGACAAATGACAAAGCCAAAAAATATCCCACCACTGACAGATAAAGAGCTGGAAAAACTCTCCCCCGAAGAGCTCAGGGCCGAACTGCGTTATCTGC
GGGCAGAGAATGCCTATCTAAAAAAGTTGAAAGCCTTGGTTCAGAGCGAAAAAAATGGCAAAAAGCCCTGATAATCAGTGAACTAAGGCATGAACACGCT
CTGCGGGACCTTCTGCGGGCGGCCGGTATGTCCCGTAGCACGTGGTATTACAATATGAATGCACTGAAGCAAGGGGACAGGTATGCGGGTCTTAAAGAGA
ACATCAGGAAGATATACCACTATCACAAAGGTCGTTATGGCTACCGCAGGATCACGCTCGCACTAAGAAAACAGGGGCTGCGGATAAACCATAAAACAGT
GCAGCGGCTGATGGCAGAACTGTCACTCCGGTCTGTGATAAGGGCGAAAAAATATCGTGCCTGGAAAGGGAGAACGGGCGAGGCCGCGCCCAATATCCTG
AGCCGGAACTTCGGTGCGTCAAAAGCCAACGAAAAATGGGTAACAGATGTGACAGAGTTCCCGGTACAGGGAAAAAAGCTTTACCTGTCGTCAGTTCTCG
ATCTGTTTAACCGGGAGGTTATCGCCTACAGCCTGTCGGAAAGGCCGGTGATGGAGATGGTGAATACGATGCTGGACGGTGCGTTCCCGAAGCTCAGACC
GGGAGATGCTCCGCTGCTGCACTCGGATCAGGGCTGGCATTACAGGATGAGGAGCTATCAGGAACGTTTAAAGGCGCATGGGATGACGCAGAGTATGTCG
CGTAAAGGAAACTGCCTGGATAATGCAGTGATGGAAAACTTCTTCGGGACCCTGAAATCGGAGTGTTTTTATCTGAGGGAGTTCAGGAGTGTCAGTGCGC
TAAGAAAAGCCGTAGAGGACTATATCCATTACTACAACAACGAGCGGATAAGCCTGAAATTAAAAGGCCTGAGTCCGGTAGAGTACCGAACCCAGGCTCT
GAGAGCCGCTTAATATGAACCATCCAACTTTATGGGGTCAGTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 522 bp | 173 aa | 50 | 571 | + | No |
Description : First part of the transposase
ORF sequence :
MAKPKYSPETKLAVVNHYLSGKDGEQSTADLFGIERTSVRRWVRAWQFHGAEGLTAKNNHYSDEFKLVVVRAVISDRLTMREAAARFNLSAEILVRRWLD
VYNDAGAEGLLNMQCGRPGQMTKPKNIPPLTDKELEKLSPEELRAELRYLRAENAYLKKLKALVQSEKNGKKP
VYNDAGAEGLLNMQCGRPGQMTKPKNIPPLTDKELEKLSPEELRAELRYLRAENAYLKKLKALVQSEKNGKKP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 966 bp | 321 aa | 448 | 1413 | + | No |
Description : Second part of the transposase
ORF sequence :
RAGKTLPRRAQGRTALSAGRECLSKKVESLGSERKKWQKALIISELRHEHALRDLLRAAGMSRSTWYYNMNALKQGDRYAGLKENIRKIYHYHKGRYGYR
RITLALRKQGLRINHKTVQRLMAELSLRSVIRAKKYRAWKGRTGEAAPNILSRNFGASKANEKWVTDVTEFPVQGKKLYLSSVLDLFNREVIAYSLSERP
VMEMVNTMLDGAFPKLRPGDAPLLHSDQGWHYRMRSYQERLKAHGMTQSMSRKGNCLDNAVMENFFGTLKSECFYLREFRSVSALRKAVEDYIHYYNNER
ISLKLKGLSPVEYRTQALRAA
RITLALRKQGLRINHKTVQRLMAELSLRSVIRAKKYRAWKGRTGEAAPNILSRNFGASKANEKWVTDVTEFPVQGKKLYLSSVLDLFNREVIAYSLSERP
VMEMVNTMLDGAFPKLRPGDAPLLHSDQGWHYRMRSYQERLKAHGMTQSMSRKGNCLDNAVMENFFGTLKSECFYLREFRSVSALRKAVEDYIHYYNNER
ISLKLKGLSPVEYRTQALRAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1364 bp | 454 aa | 50 | 1413 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKPKYSPETKLAVVNHYLSGKDGEQSTADLFGIERTSVRRWVRAWQFHGAEGLTAKNNHYSDEFKLVVVRAVISDRLTMREAAARFNLSAEILVRRWLD
VYNDAGAEGLLNMQCGRPGQMTKPKNIPPLTDKELEKLSPEELRAELRYLRAENAYLKKVESLGSERKKWQKALIISELRHEHALRDLLRAAGMSRSTWY
YNMNALKQGDRYAGLKENIRKIYHYHKGRYGYRRITLALRKQGLRINHKTVQRLMAELSLRSVIRAKKYRAWKGRTGEAAPNILSRNFGASKANEKWVTD
VTEFPVQGKKLYLSSVLDLFNREVIAYSLSERPVMEMVNTMLDGAFPKLRPGDAPLLHSDQGWHYRMRSYQERLKAHGMTQSMSRKGNCLDNAVMENFFG
TLKSECFYLREFRSVSALRKAVEDYIHYYNNERISLKLKGLSPVEYRTQALRAA
VYNDAGAEGLLNMQCGRPGQMTKPKNIPPLTDKELEKLSPEELRAELRYLRAENAYLKKVESLGSERKKWQKALIISELRHEHALRDLLRAAGMSRSTWY
YNMNALKQGDRYAGLKENIRKIYHYHKGRYGYRRITLALRKQGLRINHKTVQRLMAELSLRSVIRAKKYRAWKGRTGEAAPNILSRNFGASKANEKWVTD
VTEFPVQGKKLYLSSVLDLFNREVIAYSLSERPVMEMVNTMLDGAFPKLRPGDAPLLHSDQGWHYRMRSYQERLKAHGMTQSMSRKGNCLDNAVMENFFG
TLKSECFYLREFRSVSALRKAVEDYIHYYNNERISLKLKGLSPVEYRTQALRAA
Blast result :
Comments
ISKpn1 is 55% aa similar to IS1397 for ORF1 and 80% aa similar to IS150 for ORF2. There is a putative frameshift window at position 520-589.
This sequence is specifically inserted into the loop of a PU with a 3bp duplication.
ISKpn1 is present in fewer than 10 copies on the chromosomes of the strain cited below.
This sequence is specifically inserted into the loop of a PU with a 3bp duplication.
ISKpn1 is present in fewer than 10 copies on the chromosomes of the strain cited below.
References
1] Wilde,C., Bachellier,S., Hofnung,M. and Clement,J.M. (2001). J.Bacteriol. 183 (15), 4395-4404