ISNiba2
- Family IS1202
- Group ISTde1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| PEYF01000009 | ND | Nitrospirae bacterium | Nitrospirae bacterium CG08 |
DNA section
IS Length : 1597 bp
Ends
IR Length : 21/28
IRL : TGTAATCCCAAAATGATAATGTCCTATTCTGCCAAGATAGAAATGTCCTA
IRR : TGTCAAGCCAAAGTAGAAATGTCCTATTGTTACCAAAATAGAAATGTCCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAGGAACATTACTG | CTTTCAAATTCCTGCTTGA | 0 |
DNA sequence
TGTAATCCCAAAATGATAATGTCCTATTCTGCCAAGATAGAAATGTCCTAATAGTAGGCTATAATGCCTTCCTTAAAAGGAGGGTATGATGGTGAGAAAG
GACATGATCGAAATGAGCAGAAGGGAGTTAACCAGGATTCATTTTATCAAACAGGCGCTGGAGAAGAAGATCACGCAGGCAAAGGCAGGTGAGCTGACTG
AGTTAAGTGATCGACAGATACGGCGGCTCATAAAGAGGATGCGGAAGGAGGGAGAGACGGGGATTTGCCATTGCTCCAGGGGTAGGCTTTCAAATCGTCG
GATACCGAAGAAAATAAAGGACAAGGTCATAAAGCTCTGCCGAGGGATATATTCAGGATTTGGTCCTACCTTCGCAAGTGAAAAGCTTTTGGAGCGAGAC
GGCATCAAGATAAGCGACGAGACCTTGCGGCTTTGGTTCAAAGAAGCAGAGATTACGTATCAGGGCCGTAAGGGAAGAAAGCACCGGCAGTGGCGGGAGC
GGAAAGAGCATATTGGCGAGCTGGTACAGATGGACGGGTCCCACCACGACTGGTTCGAGGGCCGGGGTCCGAAATGTGTGCTCATGTCGTACATTGATGA
CGCCACCGGAAGGGTCTACGCCCGTTTTTACGATTATGAGGGGACCTATCCGGCCATGGACAGTTTTTGGCGCTATATCGAGAAATACGGCATGCCCCAG
AGCGTGTATTTGGACAGGCACTCAACCTATAAGTCAACGGCCAAGCCGACGATTGAGGAGGAGCTTTCAGGCAGGGATCCTCAAAGCCAGTTTGAGCGGG
CGCTTTCCGAGCTTACCGTAAAGGTCAGACATGCCGGTTCCCCCCAGGCAAAAGGCCGTATCGAGAGATTGTTTCGGACCTTTCAAGACCGGTTGGTCAA
GGATATGCGGCTTGAGGGGATCAAGACCAAGGAAGAGGCGAATCGGTTTATAGACAACGGCTACCTCAAGAAACACAACGAGAAGTTTTGCAACGATCCG
GCAAAGGAGGCCGATTTGCACCGCCCAAACCCCGGCAAAAGGATTTTGGCGGGCATCCTATGCATCAAGATCGAGCGCACCGTGCGCAATGATTTCACCA
TTGCCCATGATACCAAGCGCTATCAGATCATCGAGAAGACCCGTGCTAAGAAAGTTTTTGTAGAGGAACGGCTTAATGGGACCATGGAGATCACCTACCA
GGGCCGGAGGCTTGCGTACAAAGAGATCGCCACGCGGCCGATCAAGAAGAATCGTAAGGAAGTCGTCCTGATGAAGGATCGGGGTCACACACCATCCAAG
GATCATCCCTGGAGGCTGCCGTATAAACCGATACGCCATGCCTTTCAAGAGGCGAACCGATCCGGCTCGGAAGGGCGATTCTCTCATTGGGAGGGTGGTA
CTTAGGTATGGATCATCTCCCAAAAACTCAAATACGGCGATCCTACGAAGTCGTTTTTGAACAAAAGATGCCTGATATGCATTTCAAACAGAAAATCACT
ACGAGCGGCAAGGGGGACATTGATCTTCTCCCTTCCGCTCCTAAAACCGGACATTTCTATTTTGGTAACAATAGGACATTTCTACTTTGGCTTGACA
GACATGATCGAAATGAGCAGAAGGGAGTTAACCAGGATTCATTTTATCAAACAGGCGCTGGAGAAGAAGATCACGCAGGCAAAGGCAGGTGAGCTGACTG
AGTTAAGTGATCGACAGATACGGCGGCTCATAAAGAGGATGCGGAAGGAGGGAGAGACGGGGATTTGCCATTGCTCCAGGGGTAGGCTTTCAAATCGTCG
GATACCGAAGAAAATAAAGGACAAGGTCATAAAGCTCTGCCGAGGGATATATTCAGGATTTGGTCCTACCTTCGCAAGTGAAAAGCTTTTGGAGCGAGAC
GGCATCAAGATAAGCGACGAGACCTTGCGGCTTTGGTTCAAAGAAGCAGAGATTACGTATCAGGGCCGTAAGGGAAGAAAGCACCGGCAGTGGCGGGAGC
GGAAAGAGCATATTGGCGAGCTGGTACAGATGGACGGGTCCCACCACGACTGGTTCGAGGGCCGGGGTCCGAAATGTGTGCTCATGTCGTACATTGATGA
CGCCACCGGAAGGGTCTACGCCCGTTTTTACGATTATGAGGGGACCTATCCGGCCATGGACAGTTTTTGGCGCTATATCGAGAAATACGGCATGCCCCAG
AGCGTGTATTTGGACAGGCACTCAACCTATAAGTCAACGGCCAAGCCGACGATTGAGGAGGAGCTTTCAGGCAGGGATCCTCAAAGCCAGTTTGAGCGGG
CGCTTTCCGAGCTTACCGTAAAGGTCAGACATGCCGGTTCCCCCCAGGCAAAAGGCCGTATCGAGAGATTGTTTCGGACCTTTCAAGACCGGTTGGTCAA
GGATATGCGGCTTGAGGGGATCAAGACCAAGGAAGAGGCGAATCGGTTTATAGACAACGGCTACCTCAAGAAACACAACGAGAAGTTTTGCAACGATCCG
GCAAAGGAGGCCGATTTGCACCGCCCAAACCCCGGCAAAAGGATTTTGGCGGGCATCCTATGCATCAAGATCGAGCGCACCGTGCGCAATGATTTCACCA
TTGCCCATGATACCAAGCGCTATCAGATCATCGAGAAGACCCGTGCTAAGAAAGTTTTTGTAGAGGAACGGCTTAATGGGACCATGGAGATCACCTACCA
GGGCCGGAGGCTTGCGTACAAAGAGATCGCCACGCGGCCGATCAAGAAGAATCGTAAGGAAGTCGTCCTGATGAAGGATCGGGGTCACACACCATCCAAG
GATCATCCCTGGAGGCTGCCGTATAAACCGATACGCCATGCCTTTCAAGAGGCGAACCGATCCGGCTCGGAAGGGCGATTCTCTCATTGGGAGGGTGGTA
CTTAGGTATGGATCATCTCCCAAAAACTCAAATACGGCGATCCTACGAAGTCGTTTTTGAACAAAAGATGCCTGATATGCATTTCAAACAGAAAATCACT
ACGAGCGGCAAGGGGGACATTGATCTTCTCCCTTCCGCTCCTAAAACCGGACATTTCTATTTTGGTAACAATAGGACATTTCTACTTTGGCTTGACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1320 bp | 439 aa | 86 | 1405 | + | No |
Chemistry : DDE
ORF sequence :
MMVRKDMIEMSRRELTRIHFIKQALEKKITQAKAGELTELSDRQIRRLIKRMRKEGETGICHCSRGRLSNRRIPKKIKDKVIKLCRGIYSGFGPTFASEK
LLERDGIKISDETLRLWFKEAEITYQGRKGRKHRQWRERKEHIGELVQMDGSHHDWFEGRGPKCVLMSYIDDATGRVYARFYDYEGTYPAMDSFWRYIEK
YGMPQSVYLDRHSTYKSTAKPTIEEELSGRDPQSQFERALSELTVKVRHAGSPQAKGRIERLFRTFQDRLVKDMRLEGIKTKEEANRFIDNGYLKKHNEK
FCNDPAKEADLHRPNPGKRILAGILCIKIERTVRNDFTIAHDTKRYQIIEKTRAKKVFVEERLNGTMEITYQGRRLAYKEIATRPIKKNRKEVVLMKDRG
HTPSKDHPWRLPYKPIRHAFQEANRSGSEGRFSHWEGGT
LLERDGIKISDETLRLWFKEAEITYQGRKGRKHRQWRERKEHIGELVQMDGSHHDWFEGRGPKCVLMSYIDDATGRVYARFYDYEGTYPAMDSFWRYIEK
YGMPQSVYLDRHSTYKSTAKPTIEEELSGRDPQSQFERALSELTVKVRHAGSPQAKGRIERLFRTFQDRLVKDMRLEGIKTKEEANRFIDNGYLKKHNEK
FCNDPAKEADLHRPNPGKRILAGILCIKIERTVRNDFTIAHDTKRYQIIEKTRAKKVFVEERLNGTMEITYQGRRLAYKEIATRPIKKNRKEVVLMKDRG
HTPSKDHPWRLPYKPIRHAFQEANRSGSEGRFSHWEGGT
Blast result :
Comments
ISNiba2 is 74% aa similar to ISNiba1.
References
1] Patricia Siguier (2020) Direct submission.
2] Probst,A.J., Ladd,B., Jarett,J.K., Geller-McGrath,D.E., Sieber,C.M., Emerson,J.B., Anantharaman,K., Thomas,B.C., Malmstrom,R.R., Stieglmeier,M., Klingl,A., Woyke,T., Ryan,M.C. and Banfield,J.F. (2018) Nat Microbiol 3 (3), 328-336.
2] Probst,A.J., Ladd,B., Jarett,J.K., Geller-McGrath,D.E., Sieber,C.M., Emerson,J.B., Anantharaman,K., Thomas,B.C., Malmstrom,R.R., Stieglmeier,M., Klingl,A., Woyke,T., Ryan,M.C. and Banfield,J.F. (2018) Nat Microbiol 3 (3), 328-336.