ISNiba4
- Family IS1202
- Group ISTde1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| MHED01000202 | ND | Nitrospirae bacterium | Nitrospirae bacterium GWD2_57_9 |
DNA section
IS Length : 1446 bp
Ends
IR Length : 34/53
IRL : TGTAATCCCAAAATGATAATGTCCTAATTCGCCAAAGTTAAAATGTCCTA
IRR : TGTCAAGACAAGCTTCCTGAGCCTGTCAAGCCAAAGTAGAAATGTCCTAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGATAATTCTGTAG | ATACTTCCGATGTATGTTA | 0 |
DNA sequence
TGTAATCCCAAAATGATAATGTCCTAATTCGCCAAAGTTAAAATGTCCTATTCCCAGTTACAATGCCTGACGTTAATCGGAGGGCATTGTGGCAGAAAAG
GACATGATCACCATGAGCAGGGAGGAAGCAAAAAGACTCCACATCATCCATCAGGCATTGAATAAGAAGATCAGTCAAACAGAGGCAGCGTCACTGATCG
ACCTCAGCGACCGGCAGATTCGGCGGATGATTAAACGGATACGGAAAGAAGGCGATGAGGGCATATGCCATCGTTCCCGGGGTAAGACATCGAATCATCG
TATTTCCAAGAAGATCAAGGACAAGACCCTCAAGCTGTTTCGGGAACAGTACAAGGACTTTAATCTTGTCCATGCCACTGAGAAAATGTTTGAAGTTCAT
GGGATAGCCTTGAGCGATGAAACGCTCAGGTTATGGCTGAAGGAAGCGGAGATCCCCTACAAGAAGCGCAAAGTGAGGAAACACCGGCAGCGGCGAGAGC
CAAGAGCCCATTTTGGCGAGCTGGTACAGATCGACGGCTCCCATCATGATTGGTTCGAGGGCCGTGGACCTATCTGCGTGTTCATGGGATACATCGATGA
CGCTACAAACACCGTGCATGGCCGTTTTTATGACTATGAAGGCACCATGCCAGCCATGGACAGCCTGAAGCGGTATATCAAGCGTTATGGCATCCCTCAA
AGCGTGTATCTGGACAAGCACTCTACATATAAATCCTGGGCCGAGCCGACCATCGAGGAACAGCTTAATGATCAGAAACCGATGAGTCATTTCGAGAAGA
GTCTGACGGCATTGGCGATAGAGGTTATCCATGCCAATTCACCCCAGGCCAAAGGCAGGGTAGAGCGGCTCTTTAAGACCCTTCAGGATCGGCTGGTACG
CGAGATGCGGCTTCTGGGGATCAAGAGCGTGGATGAGGCAAACGAGTTCCTCATAACCTATCTGCCGAAGTACAACAAAAAGTTTAAAAAAGAAGCGGTA
TACGAGGTGGATCTGCACCGGCCCGCACTGCACAGCAGAGAACTCGATCGGATTCTCTGCATCAAAGAAGAGCGGACGGTAAAAAACGACTTCACCATCG
CCTATAAAAATACACTCTATCAGATCGAACAGGCGACACAGGCGAAGAAAGTCATGGTGGAAGAACGGCTCGACGGCACCTTGCACATAACCTACAACGG
GCAGGACCTGAGCTTCCGGGAAATCACGAATAAGCTGGCCAGGGACACGGCGGAAGCACCGCTCCTACTGAAAGAGAAAAAACCATGGACGCCTCCGGTT
GACCATCCTTGGAAGAAGCTCTTTCTATCAAAGAGAAAAAAACGGGAACAGCCTGTTGCTGCTCCTTAAAAACCGGACATTTCTATTTTGGTAATAATAG
GACATTTCTACTTTGGCTTGACAGGCTCAGGAAGCTTGTCTTGACA
GACATGATCACCATGAGCAGGGAGGAAGCAAAAAGACTCCACATCATCCATCAGGCATTGAATAAGAAGATCAGTCAAACAGAGGCAGCGTCACTGATCG
ACCTCAGCGACCGGCAGATTCGGCGGATGATTAAACGGATACGGAAAGAAGGCGATGAGGGCATATGCCATCGTTCCCGGGGTAAGACATCGAATCATCG
TATTTCCAAGAAGATCAAGGACAAGACCCTCAAGCTGTTTCGGGAACAGTACAAGGACTTTAATCTTGTCCATGCCACTGAGAAAATGTTTGAAGTTCAT
GGGATAGCCTTGAGCGATGAAACGCTCAGGTTATGGCTGAAGGAAGCGGAGATCCCCTACAAGAAGCGCAAAGTGAGGAAACACCGGCAGCGGCGAGAGC
CAAGAGCCCATTTTGGCGAGCTGGTACAGATCGACGGCTCCCATCATGATTGGTTCGAGGGCCGTGGACCTATCTGCGTGTTCATGGGATACATCGATGA
CGCTACAAACACCGTGCATGGCCGTTTTTATGACTATGAAGGCACCATGCCAGCCATGGACAGCCTGAAGCGGTATATCAAGCGTTATGGCATCCCTCAA
AGCGTGTATCTGGACAAGCACTCTACATATAAATCCTGGGCCGAGCCGACCATCGAGGAACAGCTTAATGATCAGAAACCGATGAGTCATTTCGAGAAGA
GTCTGACGGCATTGGCGATAGAGGTTATCCATGCCAATTCACCCCAGGCCAAAGGCAGGGTAGAGCGGCTCTTTAAGACCCTTCAGGATCGGCTGGTACG
CGAGATGCGGCTTCTGGGGATCAAGAGCGTGGATGAGGCAAACGAGTTCCTCATAACCTATCTGCCGAAGTACAACAAAAAGTTTAAAAAAGAAGCGGTA
TACGAGGTGGATCTGCACCGGCCCGCACTGCACAGCAGAGAACTCGATCGGATTCTCTGCATCAAAGAAGAGCGGACGGTAAAAAACGACTTCACCATCG
CCTATAAAAATACACTCTATCAGATCGAACAGGCGACACAGGCGAAGAAAGTCATGGTGGAAGAACGGCTCGACGGCACCTTGCACATAACCTACAACGG
GCAGGACCTGAGCTTCCGGGAAATCACGAATAAGCTGGCCAGGGACACGGCGGAAGCACCGCTCCTACTGAAAGAGAAAAAACCATGGACGCCTCCGGTT
GACCATCCTTGGAAGAAGCTCTTTCTATCAAAGAGAAAAAAACGGGAACAGCCTGTTGCTGCTCCTTAAAAACCGGACATTTCTATTTTGGTAATAATAG
GACATTTCTACTTTGGCTTGACAGGCTCAGGAAGCTTGTCTTGACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1281 bp | 426 aa | 89 | 1369 | + | No |
Chemistry : DDE
ORF sequence :
MAEKDMITMSREEAKRLHIIHQALNKKISQTEAASLIDLSDRQIRRMIKRIRKEGDEGICHRSRGKTSNHRISKKIKDKTLKLFREQYKDFNLVHATEKM
FEVHGIALSDETLRLWLKEAEIPYKKRKVRKHRQRREPRAHFGELVQIDGSHHDWFEGRGPICVFMGYIDDATNTVHGRFYDYEGTMPAMDSLKRYIKRY
GIPQSVYLDKHSTYKSWAEPTIEEQLNDQKPMSHFEKSLTALAIEVIHANSPQAKGRVERLFKTLQDRLVREMRLLGIKSVDEANEFLITYLPKYNKKFK
KEAVYEVDLHRPALHSRELDRILCIKEERTVKNDFTIAYKNTLYQIEQATQAKKVMVEERLDGTLHITYNGQDLSFREITNKLARDTAEAPLLLKEKKPW
TPPVDHPWKKLFLSKRKKREQPVAAP
FEVHGIALSDETLRLWLKEAEIPYKKRKVRKHRQRREPRAHFGELVQIDGSHHDWFEGRGPICVFMGYIDDATNTVHGRFYDYEGTMPAMDSLKRYIKRY
GIPQSVYLDKHSTYKSWAEPTIEEQLNDQKPMSHFEKSLTALAIEVIHANSPQAKGRVERLFKTLQDRLVREMRLLGIKSVDEANEFLITYLPKYNKKFK
KEAVYEVDLHRPALHSRELDRILCIKEERTVKNDFTIAYKNTLYQIEQATQAKKVMVEERLDGTLHITYNGQDLSFREITNKLARDTAEAPLLLKEKKPW
TPPVDHPWKKLFLSKRKKREQPVAAP
Blast result :
Comments
ISNiba4 is 73% aa similar to ISNiba1.
References
1] Patricia Siguier (2020) Direct submission.
2] Anantharaman,K., Brown,C.T., Hug,L.A., Sharon,I., Castelle,C.J., Probst,A.J., Thomas,B.C., Singh,A., Wilkins,M.J., Karaoz,U., Brodie,E.L., Williams,K.H., Hubbard,S.S. and Banfield,J.F. (2016)Nat Commun 7, 13219.
2] Anantharaman,K., Brown,C.T., Hug,L.A., Sharon,I., Castelle,C.J., Probst,A.J., Thomas,B.C., Singh,A., Wilkins,M.J., Karaoz,U., Brodie,E.L., Williams,K.H., Hubbard,S.S. and Banfield,J.F. (2016)Nat Commun 7, 13219.