ISAon1
- Family ISL3
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP107205.1 | ND | Alistipes onderdonkii | Alistipes onderdonkii KR001_HAM_0061 Phocaeicola vulgatus VIC01 Bacteroides stercoris ATCC 43183 |
DNA section
IS Length : 1419 bp
Ends
IR Length : 20/24
IRL : GGGTCAATGTAAAAAGCTGAGGGGTAAATTTATAAATTGTTATCTTTGTC
IRR : GGGTCAACGTAACAATCTGAGGGATTTTATATTTAAGCATATAATTTAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAAGACAGAATTTGAATCCAAT | TAAATTTC | GCTTTAAATAGAATCCTATACG | 8 |
| ATATACGCCGCTTGCGCGACAA | GACAAAGG | TAAAAAATATTTCGCAATTGAC | 8 |
| ATCCTATTTTCATGGTTTGTGATCCGATAG | ATGGATAGGATATAATACAGATATAGCAAA | 0 | |
| GCCGATGTACCCTTATCTGCTT | CTCCTTTC | CCCATCCGTACCGCCATGCGAT | 8 |
| AGAACTATCGTCAGTTTATGAA | TAAATTTC | ATCATGATTGGATCGTTAGTTT | 8 |
DNA sequence
GGGTCAATGTAAAAAGCTGAGGGGTAAATTTATAAATTGTTATCTTTGTCTCCAAATTCATAGCTTATGGAGTTAAACGGTTATCGCCTTCTTCTTCCTG
AAGGCACTTTAGATTACTTTGATCTTGTTGATGTGAAGGAAAGCGTAAATGAAGTTGTGATCTATCTTGAGGAGAAAAATATCGTTCCTGAGAAGTATAC
AGATCAGGATACTGAGAGTAAAGGCTTTTATGATCCTGTTATTGTTCAGGATTTTCCTCTTCGTGGCAAGAAGGTCTTCCTTAATATTCGTCGTCGTCGC
TGGCTCTTAAAGAAGCATAATGAGTATATCTCTCGTAACTGGCGTATGGTGGCTGAAGGTACTCGTATGACGCAAGATTTTGCGTCTTTTTTAAAAGAAC
TATATTGATAGCTATCCGATCAGTTGCAAGCTCTTAGGTGTTTTATCTGGAGTTGACGGGGATTTATTGGAGCGTCAATACCGTAACCATCTAAGTGGTT
ATCTCCATTGGGACCAACTTGTTCATGCAGAGGATTGGCTACTATTCGAAAAGAATATCGGTGCTTATATCTGTATTGATGAAGTAGCCCTCTCGCGTGG
GGAACTCTATACTGTACTGACCAATAAAGAAGCTCACGGTGGTAAAGGTAGTATGATTGCTATCATTAAAGGTACGGACGTTCATACGGTCACTTCTGTC
CTGCTTAAGCTATCCCGTCGCCGCCGTTACCAAGTGCGCGAGATTACTTTGGATATGGCTCCTAACATGGAGCAGATTGCACGCATCTGCTTTCCTGCGG
CCAAACGTGTTACCGATCGCTTTCACGTACAGAAGTTAGCTTATGAAGCGGTGCAGGAGATGCGGGTAAAAGCTCGCTGGGAGGCTTTGGATGAAGAGTC
CACTCAGATTGCTTATGCAAAGGCCTGTGGAAAGATGTATCATGCACCTGTTTTCGCTAATGGAGATACAAGAAAGCAGTTGTTAGCGAGAAGTATTTAT
CTGCTTTACAAGAAGGAATCCTTATGGACTCAGTCTCAAAGAATACGGGCTGAAATTCTTTTCAAGGAATATCCCGATATAAAGAAAGGATATTATCTGT
CTATGCGGTTAGGCTTAATCTATCATCAGTGTAAGTTTAAGGATATTGCTTTGACAAGGCTGGCAAGGTGGTATGATGAAGTAGATAAATCAGGGTTCCT
CACCTTTGGAAGGGTCGCACGTTCTATACAAACACATTATCTGGATATAATCAATTTTTTTGAAAGGAGGGCAACCAATGCCGCAGCAGAGTCATTCAAT
GCTAAAATCAAAGCATTTAGAGCACAGTTCAGAGGAGTAAGAGATAGAGCTTTTTTCTTATATAGACTTGCTAAATTATATGCTTAAATATAAAATCCCT
CAGATTGTTACGTTGACCC
AAGGCACTTTAGATTACTTTGATCTTGTTGATGTGAAGGAAAGCGTAAATGAAGTTGTGATCTATCTTGAGGAGAAAAATATCGTTCCTGAGAAGTATAC
AGATCAGGATACTGAGAGTAAAGGCTTTTATGATCCTGTTATTGTTCAGGATTTTCCTCTTCGTGGCAAGAAGGTCTTCCTTAATATTCGTCGTCGTCGC
TGGCTCTTAAAGAAGCATAATGAGTATATCTCTCGTAACTGGCGTATGGTGGCTGAAGGTACTCGTATGACGCAAGATTTTGCGTCTTTTTTAAAAGAAC
TATATTGATAGCTATCCGATCAGTTGCAAGCTCTTAGGTGTTTTATCTGGAGTTGACGGGGATTTATTGGAGCGTCAATACCGTAACCATCTAAGTGGTT
ATCTCCATTGGGACCAACTTGTTCATGCAGAGGATTGGCTACTATTCGAAAAGAATATCGGTGCTTATATCTGTATTGATGAAGTAGCCCTCTCGCGTGG
GGAACTCTATACTGTACTGACCAATAAAGAAGCTCACGGTGGTAAAGGTAGTATGATTGCTATCATTAAAGGTACGGACGTTCATACGGTCACTTCTGTC
CTGCTTAAGCTATCCCGTCGCCGCCGTTACCAAGTGCGCGAGATTACTTTGGATATGGCTCCTAACATGGAGCAGATTGCACGCATCTGCTTTCCTGCGG
CCAAACGTGTTACCGATCGCTTTCACGTACAGAAGTTAGCTTATGAAGCGGTGCAGGAGATGCGGGTAAAAGCTCGCTGGGAGGCTTTGGATGAAGAGTC
CACTCAGATTGCTTATGCAAAGGCCTGTGGAAAGATGTATCATGCACCTGTTTTCGCTAATGGAGATACAAGAAAGCAGTTGTTAGCGAGAAGTATTTAT
CTGCTTTACAAGAAGGAATCCTTATGGACTCAGTCTCAAAGAATACGGGCTGAAATTCTTTTCAAGGAATATCCCGATATAAAGAAAGGATATTATCTGT
CTATGCGGTTAGGCTTAATCTATCATCAGTGTAAGTTTAAGGATATTGCTTTGACAAGGCTGGCAAGGTGGTATGATGAAGTAGATAAATCAGGGTTCCT
CACCTTTGGAAGGGTCGCACGTTCTATACAAACACATTATCTGGATATAATCAATTTTTTTGAAAGGAGGGCAACCAATGCCGCAGCAGAGTCATTCAAT
GCTAAAATCAAAGCATTTAGAGCACAGTTCAGAGGAGTAAGAGATAGAGCTTTTTTCTTATATAGACTTGCTAAATTATATGCTTAAATATAAAATCCCT
CAGATTGTTACGTTGACCC
Recoding section
- Recoding by NULL
- Frame NULL
- Type NULL
- Experimentally demonstrated NULL
Stimulators :
- Shine-Dalgarno sequence : NULL
- Secondary structure : NULL
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 342 bp | 113 aa | 67 | 408 | + | No |
Description : First part of the transposase
ORF sequence :
MELNGYRLLLPEGTLDYFDLVDVKESVNEVVIYLEEKNIVPEKYTDQDTESKGFYDPVIVQDFPLRGKKVFLNIRRRRWLLKKHNEYISRNWRMVAEGTR
MTQDFASFLKELY
MTQDFASFLKELY
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 993 bp | 330 aa | 395 | 1387 | + | No |
Description : Second part of the transposase
ORF sequence :
KNYIDSYPISCKLLGVLSGVDGDLLERQYRNHLSGYLHWDQLVHAEDWLLFEKNIGAYICIDEVALSRGELYTVLTNKEAHGGKGSMIAIIKGTDVHTVT
SVLLKLSRRRRYQVREITLDMAPNMEQIARICFPAAKRVTDRFHVQKLAYEAVQEMRVKARWEALDEESTQIAYAKACGKMYHAPVFANGDTRKQLLARS
IYLLYKKESLWTQSQRIRAEILFKEYPDIKKGYYLSMRLGLIYHQCKFKDIALTRLARWYDEVDKSGFLTFGRVARSIQTHYLDIINFFERRATNAAAES
FNAKIKAFRAQFRGVRDRAFFLYRLAKLYA
SVLLKLSRRRRYQVREITLDMAPNMEQIARICFPAAKRVTDRFHVQKLAYEAVQEMRVKARWEALDEESTQIAYAKACGKMYHAPVFANGDTRKQLLARS
IYLLYKKESLWTQSQRIRAEILFKEYPDIKKGYYLSMRLGLIYHQCKFKDIALTRLARWYDEVDKSGFLTFGRVARSIQTHYLDIINFFERRATNAAAES
FNAKIKAFRAQFRGVRDRAFFLYRLAKLYA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1321 bp | 439 aa | 67 | 1387 | + | Yes |
Chemistry : Unknow
ORF sequence :
MELNGYRLLLPEGTLDYFDLVDVKESVNEVVIYLEEKNIVPEKYTDQDTESKGFYDPVIVQDFPLRGKKVFLNIRRRRWLLKKHNEYISRNWRMVAEGTR
MTQDFASFLKNYIDSYPISCKLLGVLSGVDGDLLERQYRNHLSGYLHWDQLVHAEDWLLFEKNIGAYICIDEVALSRGELYTVLTNKEAHGGKGSMIAII
KGTDVHTVTSVLLKLSRRRRYQVREITLDMAPNMEQIARICFPAAKRVTDRFHVQKLAYEAVQEMRVKARWEALDEESTQIAYAKACGKMYHAPVFANGD
TRKQLLARSIYLLYKKESLWTQSQRIRAEILFKEYPDIKKGYYLSMRLGLIYHQCKFKDIALTRLARWYDEVDKSGFLTFGRVARSIQTHYLDIINFFER
RATNAAAESFNAKIKAFRAQFRGVRDRAFFLYRLAKLYA
MTQDFASFLKNYIDSYPISCKLLGVLSGVDGDLLERQYRNHLSGYLHWDQLVHAEDWLLFEKNIGAYICIDEVALSRGELYTVLTNKEAHGGKGSMIAII
KGTDVHTVTSVLLKLSRRRRYQVREITLDMAPNMEQIARICFPAAKRVTDRFHVQKLAYEAVQEMRVKARWEALDEESTQIAYAKACGKMYHAPVFANGD
TRKQLLARSIYLLYKKESLWTQSQRIRAEILFKEYPDIKKGYYLSMRLGLIYHQCKFKDIALTRLARWYDEVDKSGFLTFGRVARSIQTHYLDIINFFER
RATNAAAESFNAKIKAFRAQFRGVRDRAFFLYRLAKLYA
Blast result :
Comments
ISAon1 is 54% aa similar (transposase) to ISMae37.
The third ORF is the possible ORFAB transposase created by +1 frameshift at nt 396. Fusion frameshift possible only within nt 395-408 (between two stop codons).
Although all five occurrences in A. onderdonkii show target site duplications (TSD), 0/3 occurrences in P. vulgatus strain VIC01 (CP043529.1) and 1/8 occurrences in B. stercoris ATCC 43183 (CP102262.1) showed TSD.
The third ORF is the possible ORFAB transposase created by +1 frameshift at nt 396. Fusion frameshift possible only within nt 395-408 (between two stop codons).
Although all five occurrences in A. onderdonkii show target site duplications (TSD), 0/3 occurrences in P. vulgatus strain VIC01 (CP043529.1) and 1/8 occurrences in B. stercoris ATCC 43183 (CP102262.1) showed TSD.
References
1] Peter Wang (2024) Direct submission.
2] Kim,C.Y., Ma,J. and Lee,I. (2022) Nat Commun.
2] Kim,C.Y., Ma,J. and Lee,I. (2022) Nat Commun.