ISVch1

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AF034434 ND Vibrio cholerae
Vibrio cholerae N16961
DNA section
IS Length : 1023 bp

Ends


IR Length : 21/24

IRL : TGTGATTCATAGACATCCTTTCAACTATACTGGGTGTCTATGGACAGAGA
IRR : TGTGATTCATAGAGATGGTTTCAATTTTCTTAGCTGAAGATCGAGCTTGT

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

TGTGATTCATAGACATCCTTTCAACTATACTGGGTGTCTATGGACAGAGAAATCCAACAAAGACTACAGTGGGTAAAAATGTATGAGGAATGTGGTGATG
CAGGCCTCGTATGTCGACGCTGTGGTATTTCCAGACCAACATTACGAAAGTGGGCTAAGCGATATAAGCAATGTGGAATCGCTGGTCTGGAAAGCCAGAG
CAGACGCCCTCATTCATCTCCAGATACCAAACTCACTGATGAGCTAAGAGCATTGATCCTTACGATGCGTGACAAACGCAATTTAGGGGCGCGACGTTTA
CAAACGGAATTAATCCGACTTCACAAAATACACCTAAGCACAGCGACACTCCATAAAGTTTTATCCGAAGCATCAGTCAAACCCATCGTAACTTACCGAC
GCAAAAAAGATTTCCAAAGATATGAGCGCCCAATTCCTGGTGATAGAGTCCAGATGGACACGTGTAAAATAGCGCCTGGAATTTACCAGTATACAGCTAT
TGATGATTGCTCTCGCTATCGGGTTCTAAGGTGCTACTCTCGGCGCACAGCAGCAAATACAGTCGACTTTATCGATTGTGTCGTGGAAGAGATGCCATTT
CCTATCCAGCGTATTCAGACGGACAGAGGGCGTGAATTCTTTGCTGAAAAAGTCCAGAAACAACTCATGATTTATGGGATCAAGTTTCGCCCAAATAAGC
CTGGCTCACCTCACTTGAATGGCAAAGTGGAACGCTCGCAGAAAACAGATAAAAGTGAGTTCTATCCGACCATAGATGTCTCTGTGGGGCTGCAAGAACT
GGATCTGCTACTAGCTGAATGGCAACACTACTACAACTGGGAACGGCCCCACAGCTCGTTAAATGGGCTAACCCCGATAGACAGGATTACTGAGATATCT
GATCAAACTCCTTTGTCTGAAGAGGTATCTCAGAACTACCAGATCAAGAAAGAGCGGTTTCAAGAGCAAAATTACAAGCTCGATCTTCAGCTAAGAAAAT
TGAAACCATCTCTATGAATCACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
978 bp325 aa401017+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDREIQQRLQWVKMYEECGDAGLVCRRCGISRPTLRKWAKRYKQCGIAGLESQSRRPHSSPDTKLTDELRALILTMRDKRNLGARRLQTELIRLHKIHLS
TATLHKVLSEASVKPIVTYRRKKDFQRYERPIPGDRVQMDTCKIAPGIYQYTAIDDCSRYRVLRCYSRRTAANTVDFIDCVVEEMPFPIQRIQTDRGREF
FAEKVQKQLMIYGIKFRPNKPGSPHLNGKVERSQKTDKSEFYPTIDVSVGLQELDLLLAEWQHYYNWERPHSSLNGLTPIDRITEISDQTPLSEEVSQNY
QIKKERFQEQNYKLDLQLRKLKPSL

 

Blast result :
Comments
ISVch1 is 40% aa similar to IS1649, IS1652, ISAni1 and ISSco1, 42% to IS5564, 41% to ISCgl1, 37% to IS1122 and 38% to IS1121.
It is located in a pathogenicity island VPI associated with epidemic and pandemic Vibrio cholerae strains.
References
1] Karaolis,D.K.R., Johnson,J.A., Bailey,C.C., Boedeker,E.C., Kaper,J.B. and Reeves,P.R. (1998) Proc. Natl. Acad. Sci. U.S.A. 95 (6), 3134-3139