ISAba1

  • Family IS4
  • Group IS10
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AY758396 ND Acinetobacter baumannii
Acinetobacter baumannii
DNA section
IS Length : 1180 bp

Ends


IR Length : 14/15

IRL : CTCTGTACACGATAAAAATAGATAACTCATTGAAATAATGTCATAATAAT
IRR : CTCTGTACACGACAAATTTCACAGAACCCTTATCCTATCAGGGTTCTGCC

Insertion site


Left flankDirect repeatRight flankDR Length
AATAATTTATGAGCTAATCATGCGATTTA9

DNA sequence

CTCTGTACACGATAAAAATAGATAACTCATTGAAATAATGTCATAATAATTGTTTTCTAACGACGAATACTATGACACATCTCAATGAGTTATATCTTAT
CTTAAACAAATATCTAAAATGGAACAAGTCACATTTAAAGTGCTTTGCGCTCATCATGCTTGTGATTATTTTAAAGCAAACATGTAATCTTTCTTCTGCA
TCTAAAGCCTTGCCCATCAAGTGCTTACCACAATCATTTTATCGACGTATGCAGCGCTTCTTTGCAGGTCAGTATTTTGATTATCGTCAAATTTCTCAGT
TGATTTTCAATATGTTTTCATTCGACCAAGTGCAACTGACTTTAGATAGAACCAATTGGAAATGGGGAAAACGAAATATTAATATCCTGATGCTCGCAAT
CGTTTATCGTGGAATAGCGATACCTATCCTTTGGACATTGCTTAATAAACGTGGAAATTCAGATACGAAAGAGCGTATTGCTTTGATTCAACGCTTTATA
GCCATTTTTGGTAAAGACCGTATTGTGAATGTGTTCGCAGACAGAGAGTTTATCGGTGAGCAGTGGTTTACATGGTTAATTGAACAAGACATCAACTTCT
GCATTCGTGTTAAAAAAACTTCATTGTCACCAATCATTTAGGAAAGAATCATAAAATTAGTGATTTATTTCGCCATCTTAAAGTTGGTCAAATTGAATGT
CGTAAACGACGGATTTTGGTTGGTCGGGTGAAACTATATATAAGTGCACTACAGTTAGAAAATGGAGAGCTTTTACTCGTCGTTTCTCCTCAGTTTAATG
CCAATGCTATTCAGGATTATGCATTACGCTGGGAAATTGAAACCTTATTCAGTTGTCTCAAAGGACGCGGGTTTAATCTTGAAAATACGCGCTTGACAGA
CCCTAGACGAGTGAAAAAATTGATTGCGGTGTTAGCTATAAGCTTCTGTTGGTGTTACTTAACGGGTGAATGGCAACATGATCAAAAAAAAGCGATAAAA
ATAAAGAAGCATGGACGACTCTCAATGAGTTTATTTCGCTATGGTTTAGACTATGTTCAAATGGCGATTCAGCGTTTAATTGGTTTTGGGAAAAAAGAAG
AGTTTAAGGAAATTTTGGCAATTTTAAGAAGGCAGAACCCTGATAGGATAAGGGTTCTGTGAAATTTGTCGTGTACAGAG
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
570 bp189 aa72641+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTHLNELYLILNKYLKWNKSHLKCFALIMLVIILKQTCNLSSASKALPIKCLPQSFYRRMQRFFAGQYFDYRQISQLIFNMFSFDQVQLTLDRTNWKWGK
RNINILMLAIVYRGIAIPILWTLLNKRGNSDTKERIALIQRFIAIFGKDRIVNVFADREFIGEQWFTWLIEQDINFCIRVKKTSLSPII

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
537 bp178 aa6261162+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

VTNHLGKNHKISDLFRHLKVGQIECRKRRILVGRVKLYISALQLENGELLLVVSPQFNANAIQDYALRWEIETLFSCLKGRGFNLENTRLTDPRRVKKLI
AVLAISFCWCYLTGEWQHDQKKAIKIKKHGRLSMSLFRYGLDYVQMAIQRLIGFGKKEEFKEILAILRRQNPDRIRVL

 

Blast result :
Comments
Putative frameshift site (AAAAAAA) located upstream of second ORF (position 612-618).
ISAba1 is 48% aa similar to ISDra1.
Additional accession number : EF120622, this sequence was submitted by Dr Haluk Vahaboglu ( Kocaeli University Tip Fakultesi, Tûrkiye).
References
1] Laurent Poirel (2004) Direct submission
2] Heritier C, Poirel L, Nordmann P. (2006).Clin Microbiol Infect. 12:123-130.
3] Kasap,M., Torol,S., Gacar,G., Budak,F. and Vahaboglu,H. (2007) Direct submission GenBank and ISfinder.