ISKpn2
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AJJI01000024 | ND | Klebsiella pneumoniae | Klebsiella pneumoniae subsp. pneumoniae DSM 30104 plasmid pKPt2 Klebsiella pneumoniae |
DNA section
IS Length : 1306 bp
Ends
IR Length : 11
IRL : taatgggATGGACTACCTACTCCCGCTGCGGTTAACTGTACGAAATGTGC
IRR : ----tctATGGACTACCTCCGTTTTGCAAGTACTTAATCTGGTTTTGGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTCTTCCAGT | TGTAAAAAGT | 0 |
DNA sequence
TAATGGGATGGACTACCTACTCCCGCTGCGGTTAACTGTACGAAATGTGCTCACCAGGAGAATCACCATGAATATCGTATTTCTGGGTATTGATCTGGCT
AAAAATGTTTTTCAGCTCTGCGGGTTAAACCAGGCCGGCAAACCGGTTTATACGAAACGAACTGGCCGAAAAGAATTGCTCCAGACGCTGGCAAATATTC
CTGCATGTCTGATTGGGATCGAAGCGTCCACCGGGGCATTTTACTGGCAGCGTGAGTTTGAGAAACTGAGGCACAAAGTAAAGGTCATCAGTCCTCAGTA
TGTAAAACCCTTTGTCCGCGGGCAAAAAAATGATGGTAATGATGCACAGGCCATCGCAGTGGCTCTGATGCAACCGACAATGCAGTTCGTGCCACCAAAA
AGCCCCGAACAGCAGGATATCCAGGCTTTACACCGGGCAAGGCAGCGTATTGTCAATCGCCGCACTGCTACAGTCTGTCAAATAAGAGGGCTGTTACTTG
ACCGGGGGAGCCCCATTGGCAGTGCTGTCTCCAGAGCTCGCCGGGCTATTCCTCTTATCCTTGAAGATGCAGAAAACGGTCTAAGTTCCCGTATGCGCAG
AACAATTGCCGAGCTCTATGAGCTCTTTAACGATCTCGGGCGTCGGATCCATTTTTTTGATAAGGAAATTGAAACAGTATTCAGGCAATCAGAAGCCTGT
CAGCGTATCGCCAGAGTTAAAGGCATTGGCCCTAAAACGGCCACGGCCATTGTTGCTGCTATTGGCAAAGGAACTGAATTTAAGAATGGCCGCCACTTTG
CTGCATGGCTGGGTCTGGTTCCACGCCAGCATTCGAGTGGCGACAGACAGGCGCTGATGAATATGATGAAAAAAGGCGACAAGCATCTGCGGACACTTTT
TATTCATGGCGCCCGCGCTGTCGTCAGGGTTGCCACGAATAACAATGATGGCCATATGAATCAGTGGGTTAACCAGTTAAAGGAACGGCGCGGATTTAAT
AAAACGACCGTGGCGGTCGCTAACAAAAACGCGCGAATAATCTGGTCGATGCTGAGAAATGAGACCGAGTATCAGGTAGTGTGAAATTAATCCCCTGCCA
GAAAAAGTTGCAGCGTACTGAGAAATGGTGACAGGTGCACCTGCACAATCGGAACCTGATTTTTATACTGGCCTCAGAGGCCGTCCAGTTGTTGAGGCGA
TTGTGTGCGAATTACCCATTTGGCACAGATTTTCCTGATGCCGGATAAATGTAAGCAACAATCCAAAACCAGATTAAGTACTTGCAAAACGGAGGTAGTC
CATAGA
AAAAATGTTTTTCAGCTCTGCGGGTTAAACCAGGCCGGCAAACCGGTTTATACGAAACGAACTGGCCGAAAAGAATTGCTCCAGACGCTGGCAAATATTC
CTGCATGTCTGATTGGGATCGAAGCGTCCACCGGGGCATTTTACTGGCAGCGTGAGTTTGAGAAACTGAGGCACAAAGTAAAGGTCATCAGTCCTCAGTA
TGTAAAACCCTTTGTCCGCGGGCAAAAAAATGATGGTAATGATGCACAGGCCATCGCAGTGGCTCTGATGCAACCGACAATGCAGTTCGTGCCACCAAAA
AGCCCCGAACAGCAGGATATCCAGGCTTTACACCGGGCAAGGCAGCGTATTGTCAATCGCCGCACTGCTACAGTCTGTCAAATAAGAGGGCTGTTACTTG
ACCGGGGGAGCCCCATTGGCAGTGCTGTCTCCAGAGCTCGCCGGGCTATTCCTCTTATCCTTGAAGATGCAGAAAACGGTCTAAGTTCCCGTATGCGCAG
AACAATTGCCGAGCTCTATGAGCTCTTTAACGATCTCGGGCGTCGGATCCATTTTTTTGATAAGGAAATTGAAACAGTATTCAGGCAATCAGAAGCCTGT
CAGCGTATCGCCAGAGTTAAAGGCATTGGCCCTAAAACGGCCACGGCCATTGTTGCTGCTATTGGCAAAGGAACTGAATTTAAGAATGGCCGCCACTTTG
CTGCATGGCTGGGTCTGGTTCCACGCCAGCATTCGAGTGGCGACAGACAGGCGCTGATGAATATGATGAAAAAAGGCGACAAGCATCTGCGGACACTTTT
TATTCATGGCGCCCGCGCTGTCGTCAGGGTTGCCACGAATAACAATGATGGCCATATGAATCAGTGGGTTAACCAGTTAAAGGAACGGCGCGGATTTAAT
AAAACGACCGTGGCGGTCGCTAACAAAAACGCGCGAATAATCTGGTCGATGCTGAGAAATGAGACCGAGTATCAGGTAGTGTGAAATTAATCCCCTGCCA
GAAAAAGTTGCAGCGTACTGAGAAATGGTGACAGGTGCACCTGCACAATCGGAACCTGATTTTTATACTGGCCTCAGAGGCCGTCCAGTTGTTGAGGCGA
TTGTGTGCGAATTACCCATTTGGCACAGATTTTCCTGATGCCGGATAAATGTAAGCAACAATCCAAAACCAGATTAAGTACTTGCAAAACGGAGGTAGTC
CATAGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1017 bp | 338 aa | 68 | 1084 | + | No |
Chemistry : DEDD
ORF sequence :
MNIVFLGIDLAKNVFQLCGLNQAGKPVYTKRTGRKELLQTLANIPACLIGIEASTGAFYWQREFEKLRHKVKVISPQYVKPFVRGQKNDGNDAQAIAVAL
MQPTMQFVPPKSPEQQDIQALHRARQRIVNRRTATVCQIRGLLLDRGSPIGSAVSRARRAIPLILEDAENGLSSRMRRTIAELYELFNDLGRRIHFFDKE
IETVFRQSEACQRIARVKGIGPKTATAIVAAIGKGTEFKNGRHFAAWLGLVPRQHSSGDRQALMNMMKKGDKHLRTLFIHGARAVVRVATNNNDGHMNQW
VNQLKERRGFNKTTVAVANKNARIIWSMLRNETEYQVV
MQPTMQFVPPKSPEQQDIQALHRARQRIVNRRTATVCQIRGLLLDRGSPIGSAVSRARRAIPLILEDAENGLSSRMRRTIAELYELFNDLGRRIHFFDKE
IETVFRQSEACQRIARVKGIGPKTATAIVAAIGKGTEFKNGRHFAAWLGLVPRQHSSGDRQALMNMMKKGDKHLRTLFIHGARAVVRVATNNNDGHMNQW
VNQLKERRGFNKTTVAVANKNARIIWSMLRNETEYQVV
Blast result :
Comments
ISKpn2 is 97% aa similar to ISSaen1.
The IR of this IS are not at its termini. In the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
The transposase gene is equivalent to rmpB.
By analogy with IS4321, ISKpn2 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
File updated: November 13 2012.
AJJI01000024 take the place of the previous sequence (1919464A). This isoform sequence encodes a complete transposase.
The IR of this IS are not at its termini. In the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
The transposase gene is equivalent to rmpB.
By analogy with IS4321, ISKpn2 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
File updated: November 13 2012.
AJJI01000024 take the place of the previous sequence (1919464A). This isoform sequence encodes a complete transposase.
References
1] Vasselon et al. (1991) Res. Microbiol. 142, 47-54
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
3] Lee,J.H., Cheon,I.S., Shim,B.S., Kim,D.W., Kim,S.W., Chun,J. and Song,M. (2012) J. Bacteriol. 194 (20), 5722-5723
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
3] Lee,J.H., Cheon,I.S., Shim,B.S., Kim,D.W., Kim,S.W., Chun,J. and Song,M. (2012) J. Bacteriol. 194 (20), 5722-5723