ISAba3
- Family IS1
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
AY665723 | ND | Acinetobacter baumannii | Acinetobacter baumannii MAD |
DNA section
IS Length : 794 bp
Ends
IR Length : 21/24
IRL : GGTAGTGTTTCAAAAAGTATGCTGAAGAAAAAGCCGATTGGATTTTGATA
IRR : GGTGGTGCTTCAAAAAGTATGCTGCCTTTAAATGAAGCCATTATTGATGT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
CTTAACATAAAATTTT | AAAAATAGATTAACCT | 0 |
DNA sequence
GGTAGTGTTTCAAAAAGTATGCTGAAGAAAAAGCCGATTGGATTTTGATAAAATAGAGAAATGCAAATAACTCTAGCAATCAAATGTCCAACCTGCCTCA
GTGATAGTATAAAGAAAAATGGTATCAAAGTAGATGGGAAACAAAACTATCAGTGCAAAGACTGTAAACGTCAGTTTATTGGTGATCATGCACTGAGCTA
TCTAGGATGTAAGTCAGGTATTACTCGTAAAATATTACAGTTGATGGTCAGAGGCAGCGGTATACGAGATATTGCTGAAGTTGAGCGCATCAGTATCGGT
AAAGTTTTACGTACTTTAACTGAATCAACCTATGAAATTCAGCCTCAGCAAAGTCATTATGAATCTCTCGAAGTAGATGAGTTCTGGAATTTTGTTGGAA
ATAAAAAGAATAAACAATGGCTTATTTACGCCTATCATCGAGAAACAGGTGAAATTGTTGCTTATGTTTGGGGTAAACGAGATTTAGCTACTGTCTAACA
ATTGAAGACAAAGCTTAAACAATTAGATATTCACTACACCCGAATTGCAAGTGATCATTGGGACAGTTTCATCACTGCTTTTAAAAATGGTAAGCAAAGT
ATTGGTAAATTTTTTACTGTAGGCATTGAAGGCAATAATTGCAAAATAAGGCATAGAATTAGGCGTAGCTTCAGAAGAAGCTGTAATTTTTCGAAAAAGC
TTGAAAACCATTTTAAAGCTTTTGATTTAGCCTTCTTTTCTTTTACATCAATAATGGCTTCATTTAAAGGCAGCATACTTTTTGAAGCACCACC
GTGATAGTATAAAGAAAAATGGTATCAAAGTAGATGGGAAACAAAACTATCAGTGCAAAGACTGTAAACGTCAGTTTATTGGTGATCATGCACTGAGCTA
TCTAGGATGTAAGTCAGGTATTACTCGTAAAATATTACAGTTGATGGTCAGAGGCAGCGGTATACGAGATATTGCTGAAGTTGAGCGCATCAGTATCGGT
AAAGTTTTACGTACTTTAACTGAATCAACCTATGAAATTCAGCCTCAGCAAAGTCATTATGAATCTCTCGAAGTAGATGAGTTCTGGAATTTTGTTGGAA
ATAAAAAGAATAAACAATGGCTTATTTACGCCTATCATCGAGAAACAGGTGAAATTGTTGCTTATGTTTGGGGTAAACGAGATTTAGCTACTGTCTAACA
ATTGAAGACAAAGCTTAAACAATTAGATATTCACTACACCCGAATTGCAAGTGATCATTGGGACAGTTTCATCACTGCTTTTAAAAATGGTAAGCAAAGT
ATTGGTAAATTTTTTACTGTAGGCATTGAAGGCAATAATTGCAAAATAAGGCATAGAATTAGGCGTAGCTTCAGAAGAAGCTGTAATTTTTCGAAAAAGC
TTGAAAACCATTTTAAAGCTTTTGATTTAGCCTTCTTTTCTTTTACATCAATAATGGCTTCATTTAAAGGCAGCATACTTTTTGAAGCACCACC
Protein section
ORF number : 1
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
735 bp | 244 aa | 61 | 795 | + | No |
Chemistry : DDE
ORF sequence :
MQITLAIKCPTCLSDSIKKNGIKVDGKQNYQCKDCKRQFIGDHALSYLGCKSGITRKILQLMVRGSGIRDIAEVERISIGKVLRTLTESTYEIQPQQSHY
ESLEVDEFWNFVGNKKNKQWLIYAYHRETGEIVAYVWGKRDLATV*QLKTKLKQLDIHYTRIASDHWDSFITAFKNGKQSIGKFFTVGIEGNNCKIRHRI
RRSFRRSCNFSKKLENHFKAFDLAFFSFTSIMASFKGSILFEAP
ESLEVDEFWNFVGNKKNKQWLIYAYHRETGEIVAYVWGKRDLATV*QLKTKLKQLDIHYTRIASDHWDSFITAFKNGKQSIGKFFTVGIEGNNCKIRHRI
RRSFRRSCNFSKKLENHFKAFDLAFFSFTSIMASFKGSILFEAP
Blast result :
Comments
The transposase does not appear to require a frameshift as other IS1 family members. There is a termination codon which interrupts the single Tpase orf. The orf carries all the expected IS1 signatures (see Ton-Hoang et al., 2004)
ISAba3 is 57% aa similar to ISEhe5.
ISAba3 is 57% aa similar to ISEhe5.
References
1] Poirel Laurent. (2004) Direct submission
2] Poirel L, Marqué S, Héritier C, Segonds C, Chabanon G, Nordmann P (2005). Antimicrob. Agents Chemother., 49 :202-208.
2] Poirel L, Marqué S, Héritier C, Segonds C, Chabanon G, Nordmann P (2005). Antimicrob. Agents Chemother., 49 :202-208.